Fig. 4

Sirt3 deficiency resulted in further up-regulation of Acod1 expression in BMDMs treated with C16:0 + MSU. a The volcano plot is used to indicate the up-regulated (in red) ribonucleic acids in the Sirt3^−/− BMDMs treated with C16:0 + MSU. Red spots represent − log10(P) values of ≥ 2. b BMDMs were stimulated with C16:0, MSU, or C16:0 + MSU for 12 h. Western blot was used to detect Acod1 protein levels. c Acod1 protein levels in PBMCs from HC, AG and IG. d The protein levels of ACOD1 in the mouse paw injected with MSU crystals or vehicle. e ITGB7, ACOD1, and CCL2 protein expression was up-regulated in Sirt3 deficient BMDMs in response to C16:0 + MSU. f and g CCL2 secretion and CCR2 protein expression were detected in Sirt3 deficient BMDMs treated with C16:0 + MSU. *P < 0.05. NS shows no statistical difference