Fig. 6
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Acod1 knockdown suppressed CCL2 and CCR2 expression. Sirt3 deficiency or Acod1 knockdown affected BMDM migration-induced C16:0 + MSU. a–c The CCL2 and CCR2 expression level was detected in BMDMs transfected siRNA for 36 h and then treated with C16:0 + MSU for 12 h. d and e BMDMs from Sirt3+/+ and Sirt3.−/− mice treated with C16:0 + MSU or BMDMs transfected with siRNA and then treated with C16:0 + MSU. Rhodamine phalloidin-labeled F-actin (red) and DAPI-labeled nucleus (blue) were analyzed by confocal microscopy. Representative cells in each group surrounded in the white frames were enlarged. Bottom panel shows line scans (along the straight white line) of fluorescence intensities with F-actin localization. A histogram chart of relative fluorescence intensity was on the right. Scar bar, 40 μm. *P < 0.05. NS indicates P > 0.05