Fig. 8
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Acod1 knockdown decreased Drp1 localization in mitochondria and FIS1 protein levels and promoted OPA1, MFN1, and MFN2 protein levels. Compound C reversed the effect of Acod1 knockdown on p-Drp1 protein levels. a Representative LCM images of BMDMs co-stained with Mito-Tracker Red probe and p-DRP1 Ab. DAPI stains nuclei. Scale bar, 20 μm. Representative cells in each group surrounded in the white frames were enlarged. A histogram chart of relative fluorescence intensity was on the right. b and c The protein levels of p-Drp1, OPA1, FIS1, MFN1, and MFN2 were detected using Western blot. *P < 0.05. NS means no statistical difference