Table 3 The primers used for quantitative PCR
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Items | Forward sequence (5′-3′) | Reverse sequence (5′-3′) |
|---|---|---|
Mice IL-1β | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
Mice Nlrc3 | CCACGAGGAAGCAAGAGGTG | TTTGTCCCGGACTTGTAGCAG |
Mice Ccl2 | CCTTTGAATGTGAAGTTGACCCG | TAAGGCATCACAGTCCGAGTCAC |
Mice iNOS | GGAGTGACGGCAAACATGACT | TCGATGCACAACTGGGTGAAC |
Mice Itgb7 | CTCAATGAAGGACGACTTGGAAC | GACAACGCTCCAGTCGGCTG |
Mice Acod1 | CCCATAGCGAACGCTGCCACT | GAAGGCACCGAACCCTGACCC |
Mice COX-2 | GGAGTGACGGCAAACATGACT | TCAGGAAGCTCCTTATTTCCCTT |
Mice TNF-α | ACTGAACTTCGGGGTGATCG | TCCACTTGGTGGTTTGTGAGT |
Mice Sirt3 | GAGCGGCCTCTACAGCAAC | GGAAGTAGTGAGTGACATTGGG |
Mice GAPDH | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |
Human Sirt3 | ACCCAGTGGCATTCCAGAC | GGCTTGGGGTTGTGAAAGAAG |
GAPDH | CTGGGCTACACTGAGCACC | AAGTGGTCGTTGAGGGCAATG |