Table 4 The antibodies were used in Western blot and immunofluorescence
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Antibody | Source | Identifier | Dilution |
|---|---|---|---|
Sirt3 | HUABIO | R1511-3 | 1:750 |
ACOD1 | Abcam | ab222411 | 1:1000 |
Acetylated-lysine antibody | Cell Signaling Technology (CST) | 9441 | 1:1500 |
Anti-SOD2/MnSOD (acetyl K68) | Abcam | ab137037 | 1:1000 |
Integrin beta 7 | Proteintech | 11,328–1-AP | 1:1000 |
NF-κB p65 | HUABIO | ET1603-12 | 1:1000 |
Phospho-NF-κB p65 (Ser536) (93H1) | Cell Signaling Technology (CST) | 3033S | 1:750 |
Phospho-DRP1 (Ser616) | Cell Signaling Technology (CST) | 3455S | 1:750 |
AMPK alpha 1 | HUABIO | ET1608-40 | 1:1000 |
Phospho-AMPKα (Thr172) (D4D6D) | Cell Signaling Technology (CST) | 2535S | 1:750 |
CCL2 | HUABIO | HA500267 | 1:1000 |
CCR2 | HUABIO | ET1611-65 | 1:1000 |
iNOS | Abcam | ab178945 | 1:1500 |
COX2/Cyclooxygenase 2 | HUABIO | ET1610-23 | 1:1500 |
NLRP3(NLRC3) | HUABIO | ET1610-93 | 1:1500 |
IL-1β (D3U3E) | Cell Signaling Technology (CST) | 12703S | 1:1000 |
Caspase-1 | Adipogene | AG-20B-0042-C100 | 1:1000 |
Myeloperoxidase (MPO) | Abcam | ab208670 | 1:100 |
ASC(WB) | Adipogene | AG-25B-0006PF-C100 | 1:1000 |
ASC(IF) | Santa Cruz Biotech | Sc-514414 | 1:50 |
Tubulin | HUABIO | ET1602-4 | 1:1500 |
GAPDH | HUABIO | HA721136 | 1:1500 |