Fig. 1

CD45 cell subsets in advanced OA synovium. A Representative example of flow cytometry plot gating lymphocytes, myeloid cells, and CD45− cells using SSC-A vs CD45. B Relative frequencies (%) of lymphocyte, myeloid, and CD45− subsets as a representation of all viable cells. n=10. C Representative immunofluorescence imaging of immune cells (CD45, cyan) in advanced OA synovium; DAPI staining (blue) was used to visualise nuclei and CD146 (orange) to visualise vessels