Fig. 2

SMAD2 affected the expression of ASC, NLRP3, Caspase-1, and GSDMD. A, B LV-SMAD2 was transfected into FLS-RA, and the expression of SMAD2 was detected by Western blot. C, D LV-SMAD2 was transfected into FLS-RA, and the expression of ASC and NLRP3 was detected in FLS-NC, FLS-RA, and LV-SMAD2. E, F FLS-RA were treated with nigericin by concentration of 10 uM for 24h accompanied with LV-SMAD2 or not, the expression of ASC, NLRP3 were detected by Western blot. G–J the expression of caspase-1, cleaved-caspase-1, GSDMD-Full, and GSDMD-N in three groups were detected by Western blot. The experiment was repeated three times and statistical data was presented as mean ± SD. In D, * means comparison between FLS-NC and FLS-RA groups. # means comparison between FLS-NC and LV-SMAD2 groups. * ,# P<0.05. In F, H, and J, * means comparison between control and nigericin groups. # means comparison between nigericin and nigericin+SMAD2 groups. * ,# P<0.05