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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Preosteoclast plays a pathogenic role in syndesmophyte formation of ankylosing spondylitis through the secreted PDGFB — GRB2/ERK/RUNX2 pathway

Fig. 4

PDGFB pathway regulation in ADSCs. A Volcano plot of RNA sequencing in ADSCs for the two groups (PDGF ( −) and PDGF ( +)). B Genes with differential expression in the MAPK pathway between the two groups. C Differential enrichment of phosphopeptides in ADSCs. MEK1, ERK1/2, and MKNK were identified as differentially phosphorylated in the MAPK pathway. D Gene and protein expression of GRB2 after transfection with siGRB2. E, F Western blot of several key molecules in the GRB2-pERK-RUNX2 axis with/without the treatments of PDGFB and si-GRB2. G, H The mineralization ability of ADSCs with/without the treatments of PDGFB and si-GRB2. I, J Western blot of several key molecules in the GRB2-pERK-RUNX2 axis with/without the treatments of PDGFB and ERK inhibitor. K, L PDGFB activated the GRB2-pERK-RUNX2 axis in a dose-dependent manner. The gene and protein levels were compared by unpaired sample t-tests. *** P < 0.005, ** P < 0.01, * P < 0.05

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Arthritis Research & Therapy

ISSN: 1478-6362

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