Fig. 3

ST2825 inhibits the pathological gene expression of LPS-stimulated RA SFs. RA SFs were stimulated with LPS to evaluate the effect of ST2825 on gene expression molecules involved in inflammation and cell cycle. A Heatmap shows the overall expression of inflammatory genes in RA SFs stimulated with LPS and LPS plus ST2825. Three biological replicates were used per each condition. Values are shown in RPKM Log2. C Downregulated catabolic genes in RA SFs treated with LPS plus ST2825 were confirmed by qRT-PCR. Two biological replicates were analyzed in triplicates to calculate FC by the 2^−ΔΔCt method, and statistical significance was calculated by Student’s t-test. B Heatmap shows the overall expression of cell cycle-related genes in RA SFs stimulated with LPS and LPS plus ST2825. Three biological replicates were used per each condition. Values are shown in RPKM Log2. D Downregulated cell cycle-related genes in RA SFs treated with LPS plus ST2825 were confirmed by qRT-PCR. Two biological replicates were analyzed in triplicates to calculate FC by the 2^−ΔΔCt method, and statistical significance was calculated by Student’s t-test. E Transcriptomic analysis identified the top five downregulated canonical pathways in RA SFs treated with LPS plus ST2825. F Interatomic gene cluster shows the main downregulated molecules controlling cell cycle progression in RA SFs treated with LPS plus ST2825. Transcriptomic analysis identified the top five upregulated canonical pathways in RA SFs treated with LPS plus ST2825. H Interatomic gene cluster shows the main upregulated molecules controlling mitochondrial processes in RA SFs treated with LPS plus ST2825. *p < 0.05, ***p < 0.001