Fig. 4
ST2825 suppresses the invasiveness of LPS-stimulated RA SFs 3D spheroids. RA SFs 3D spheroids were performed and stimulated with LPS to potentiate their invasive capacity on the Matrigel matrix. A To monitor the long-term effect of ST2825 suppressing invasiveness, spheroids were evaluated at 48, 72, and 96 h. Three biological replicates with at least three technical replicates per sample were used. The line graph shows the invaded area in mm2. Mixed-effects model restricted maximum likelihood (REML) and Tukey’s multiple comparisons were implemented for the statistical analysis. *p < 0.01, untreated vs. LPS (96 h); φ p < 0.01, LPS vs. LPS + ST2825 (96 h). B The fold change for the area of invasion was calculated for individual values considering untreated cells as the control group. Significant suppression of invasiveness in LPS-stimulated RA SFs 3D spheroids treated with ST2825 at 96 h was observed. One-way ANOVA and Tukey’s multiple comparisons test were performed. **p < 0.01. C Representative image shows the differences in invasion area quantification (scale bar = 200 μm) among conditions at 0, 48, 72, and 96 h