Fig. 1

CUR reduces CRAMP and Calprotectin levels in the joints of CIA mice. Joint tissues obtained from four different groups of mice, CIA mice, saline control mice, CIA mice with oral administration of CUR, and CUR alone control mice (n = 7 per group) were homogenized. Joint tissue lysates (25 μg protein each) were resolved on NuPage 4–12% Bis–Tris protein gels and probed in immunoblots to assess the abundance of mouse CRAMP, S100A8, and S100A9. Antibody for β-actin was used to assess loading control. A. Densitometry for band intensity was determined using the AmershamTM Imager 680 analysis software version 2.0. The relative band intensity was determined by normalizing to the β-actin band intensity for each sample. The GraphPad Prism 9 software was used for statistical analyses. One-way ANOVA with Tuckey’s post hoc multiple comparison analysis was used to determine the p-values (*p ≤ 0.05, **p ≤ 0.005). B. A western blot image representative of data obtained from n = 7 mice per group