Table 2 M6A methylation detection methods
Methods | Characteristics | Resolution ratio | Technical shortcoming |
|---|---|---|---|
MeRIP-seq/m6A-seq | Easy to do by using m6A antibody | 200 nt | Relatively quantitative, low resolution, nonspecific binding |
PA-m6A-seq | 4-sU and RNase T1, high resolution | 30 nt | Relatively quantitative, incomplete detection |
M6A-CLIP/miCLIP | 254Â nm UV crosslinking | single base | Relatively quantitative, high RNA consumption |
NT-m6A-seq | Nanopore technology, low capture error rate, and high accuracy of base recognition | single base | Relatively quantitative, high RNA consumption |
MAZTER-seq | MazF RNA enzyme, quantitative analysis | single base | Limited applicable objects |
DART-seq | Cytidine deaminase APOBEC1, quantitative analysis | single base | Complex operation |