Fig. 3

The pro-inflammatory cytokines in liver and kidney were increased in TNF-Tg mice. A–E The gene expression of inflammatory factors including TNF-α (A) (statistics: ***p = 0.0002; *p = 0.0163; ***p = 0.0008), IL-10 (B) (statistics: ***p = 0.0004; **p = 0.0054; **p = 0.0059), IL-1β (C) (statistics: **p = 0.0048; *p = 0.0309; *p = 0.0357), and IL-6 (D) (statistics: ***p = 0.0002; *p = 0.0166) were significantly elevated in the liver of TNF-Tg mice at different time points, while TGF-β (E) was not significantly different. F–J Intrarenal inflammatory factors TNF-α (F, statistics: *p = 0.0434; *p = 0.0369; *p = 0.0220) and IL-10 (G, statistics: **p = 0.0099; **p = 0.0011; *p = 0.0220) were significantly elevated at different time points; IL-1β (H, statistics: **p = 0.0092) and TGF-β (G, statistics: *p = 0.0297) were significantly elevated at 5.5 months of age; and IL-6 (I, statistics: *p = 0.0340) was significantly increased at 3.5 months of age