Fig. 6

Anti-TNF therapy reduced immunocyte accumulation and pro-inflammatory cytokines in liver and kidney in TNF-Tg mice. A Representative images of the glomerulus podocyte and glomerulus mesangial of mice (scale bar, 50 μm). B–C Quantification of the percentage of Nephrin (Statistics: ***p = 0.0003; **p = 0.0019) (B) and NG2 (Statistics: **p = 0.0010; *p = 0.0119)) (C) in glomeruli. D Representative images of immune cells in the liver of 4.5-month-old female TNF-Tg mice (scale bar, 50 μm). E–F Quantification of the number of immune cells including CD45R positive B cells (E, statistics: ***p = 0.0010; *p = 0.0132; **p = 0.0085) and F4/80 iNOS double positive macrophage (F, statistics: **p = 0.0044; *p = 0.0110; ns = 0.0909) in the liver of TNF-Tg mice were significantly increased compared to their matched WT littermates, which were significantly reduced after anti-TNF treatment. G–I The gene expression of inflammatory factors in liver including IL-10 (G) (statistics: **p = 0.0035; *p = 0.0195), TNF-α (H) (statistics: ****p < 0.0001; **p = 0.0020), and TGF-β (I) (statistics:**p = 0.0020; **p = 0.0031) were significantly elevated in TNF-Tg mice and decreased after anti-TNF treatment. J-L The gene expression of inflammatory factors in kidney including IL-10 (J) (statistics: ****p < 0.0001****p < 0.0001), TNF-α (K) (statistics: **p = 0.0022; **p = 0.0084), and TGF-β (L) (statistics: ***p = 0.0004; **p = 0.0027) were significantly elevated in TNF-Tg mice and decreased after anti-TNF treatment