Isotype | Anti-MAA+ RA patients %a | Difference protocolsb |
|---|
Main | 1st | 2nd | p main vs. 1st | p main vs. 2nd |
|---|
IgG | 14.6 | 14.9 | 8.3 | 0.7 | 0.4 |
IgM | 6.3 | 8.3 | - | 1 | - |
IgA | 6.3 | 4.2 | - | 1 | - |
- The main protocol was used in Fig. 1 and Table 1. The 1st modified protocol changed the HSA source, increased the ratio of malondialdehyde to acetaldehyde to 4:1, replaced BSA for casein in the blocking buffer and the adduct purification system. The 2nd modified protocol was as the 1st except for the 2:1 MDA to AA ratio and the anti-Fcγ secondary antibody instead of the anti-(H + L) IgG antibody
- aPatients and controls, 48 and 48, were randomly selected from the Santiago collection. The cut-off for positivity was specific for each protocol at the estimated 98th percentile
- bComparison of the protocols with the McNemar test (with the Yates correction) for paired samples
