Fig. 2

Mapping effects of CBX4 (wild-type and mutants) on primary human chondrocytes. Expression of senescence markers and cell proliferation of primary human chondrocytes transduced by CBX4 wild-type (WT) or mutants. A CBX4 gene expression (n = 6) and B protein expression by Western blot (representative blot with quantification of n = 5 biological replicates in dot plot) in cells transduced by the empty vector Control (Ctrl), CBX4 WT, or its mutants. The expected molecular weights of CBX4 WT, CBX4 CDM, CBX4 ΔSIMs, CBX4 ΔC-box, and CBX4 CDMΔSIM were 75 kDa, 75 kDa, 74 kDa, 72 kDa, and 74 kDa, respectively. C Protein-level expression of senescence markers DPP4, SA-β-gal activity, and p16.^INK4A and cell proliferation marker EdU quantified by flow cytometry and plotted as a dot bar graph. Repeated measures with Dunnett’s post hoc test were performed to compare the effects of CBX4 WT and mutants. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001