Table 1 CBX4-related lentiviral constructs used for viral transduction of primary human chondrocytes
Created vector | Vector ID | Vector name | Size | Function of domain |
|---|---|---|---|---|
Wild-type CBX4 (CBX4 WT) | VB-210801-1043rpz | pLV[Exp]-Puro-CMV > hCBX4[NM_003655.3] | 9588 bp |  |
CDM-mutated CBX4 (CBX4 CDM) | VB-210709-1084wpn | pLV[Exp]-Puro-CMV > hCBX4*(F11A, W35L) | 9588 bp | PRC1-related chromatin modification |
SIM-deleted CBX4 (CBX4 ΔSIMs) | VB-210709-1077tkm | pLV[Exp]-Puro-CMV > hCBX4*(delete 262–265, 462-465aa) | 9564 bp | SUMO E3 ligase function |
C-box-deleted CBX4 (CBX4 ΔC-box) | VB-210709-1085knf | pLV[Exp]-Puro-CMV > hCBX4*(delete 531-556aa) | 9510 bp | Transcriptional silencing function of pcG proteins |
CDM-mutated and SIM-deleted CBX4 (CBX4 CDMΔSIM) | VB-210709-1088mgj | pLV[Exp]-Puro-CMV > hCBX4* (F11A, W35L; delete 252–265,462-465aa) | 9564 bp |  |
Control (Ctrl) | VB-210808-1067mmj | pLV[Exp]-Puro-CMV | 7899Â bp | Â |
mCherry control | VB010000-9298rtf | pLV[Exp]-EGFP:T2A:Puro-EF1A > mCherry | 10085 bp |  |