Fig. 4

GMSC home to the site of implantation and induce RASF PCD. A Histogram overlay showing the ViaFluor 405-positive cells harvested from the implanted cartilage in the 5 mice injected with labeled GMSC. Negative controls show no positive cells, while labeled GMSC maintained in culture show positive cells over the 4-day in vivo experiment. B In vitro experiment showing % RASF positive for AnnexinV. Co-incubation of RASF to GMSC at 1:1 ratio significantly increased the occurrence of AnnexinV-positive RASF, while a 10:1 ratio was no different from RASF alone (mean ± SE, n = 3)