Fig. 5

GMSC transfer molecular cargo in vitro. In vitro co-incubation of GMSC with RASF shows transfer of the intracellular label ViaFluor488 from the GMSC to the RASF when incubated together in wells with no separation, A and B, or in transwells where cells are separated, C and D. The label is transferred in a ratio-dependent manner in which increasing the GMSC:RASF ratio increases the number of RASF that become positive for the GMSC label, B and D. Data are representative of two experiments performed in triplicate