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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: ACSL4 inhibition prevents macrophage ferroptosis and alleviates fibrosis in bleomycin-induced systemic sclerosis model

Fig. 4

Calpain knockdown downregulates ACSL4 expression and rescues M1 ferroptosis. A, B Western blots of Calpain system in Raw264.7 treated with PBS or LPS. C Relative mRNA levels of Calpain 1 and Calpain 2 in Raw264.7 after CAPN1 or CAPN2 knockdown. D–G Western blots of Calpain 1, Calpain 2, and ACSL4 in Raw264.7 after CAPN1 or CAPN2 knockdown. H Relative mRNA levels of ACSL4 in Raw264.7 after CAPN1 or CAPN2 knockdown. I, J Raw264.7 cell viability after treated with erastin of different duration or concentration detected by CCK8. K–N Western blots of ferroptosis markers in Raw264.7 after CAPN1 or CAPN2 knockdown. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001)

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Arthritis Research & Therapy

ISSN: 1478-6362

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