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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: ACSL4 inhibition prevents macrophage ferroptosis and alleviates fibrosis in bleomycin-induced systemic sclerosis model

Fig. 5

ACSL4 inhibition abolishes overshooting Calpain-mediated ferroptosis. A–D Western blots for Calpain system and ACSL4 in Raw264.7 after CAPN1 or CAPN2 over-expression. E Relative mRNA levels of calpain 1 and calpain 2 in Raw264.7 after CAPN1 or CAPN2 over-expression. F–I Western blots for ferroptosis markers in Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. J, K Relative mRNA levels of ferroptosis markers in Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. L, M Relative MDA levels and net GSH concentrations of Raw264.7 after CAPN1 or CAPN2 over-expression with rescued by ROSI (1 μM, 24 h) or not. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001, * stands for lv-control versus lv-over-expression group; #p < 0.05, ##p < 0.01, ###p < 0.001, #stands for control group versus ROSI incubated group)

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Arthritis Research & Therapy

ISSN: 1478-6362

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