Fig. 5
From: Bruton’s tyrosine kinase is a possible therapeutic target in microscopic polyangiitis
Tirabrutinib did not affect MPO-ANCA production but reduced NET formation in MPA model rats. MPO-ANCA titer in MPA model rats was quantified by ELISA chronologically (a). NET-forming neutrophils in the blood were counted by FCM using Sytox Green at the end of the study (b). Five rats in the control (drug-free) group, six rats in each preadministration group (low and high doses), three rats in the low-dose postadministration group, and four rats in the high-dose postadministration group were randomly selected and subjected to this assay. *p < 0.05; ***p < 0.005. Immunofluorescent staining for NETs in the kidneys (c). NETs were identified as Cit-H3, MPO, and DAPI-positive substances (arrowheads). Dotted circles indicate glomeruli. Bar, 50 µm