Fig. 3

Th1-like Tregs were increased by IFN-α under in-sequence condition. CD4⁺CD25⁺Tregs purified from PBMCs of SLE patients were stimulated under TCR, out-of-sequence or in-sequence condition for 5 days, then analyzed using flow cytometry. A Percentages of CXCR3⁺Th1 cells, Foxp3⁺Tregs, and T-bet⁺Foxp3⁺Th1-like Tregs. B Percentages of T-bet⁺Th1 cells and T-bet⁺Foxp3⁺Th1-like Tregs. C Levels of T-bet, CXCR3, Foxp3, and CD25 expressed in MFI. D Percentages of TIGIT⁺ cells in Foxp3⁺Tregs. Data are mean ± SEM of five independent experiments using different donors. *P < 0.05, **P < 0.01