Fig. 6

TIGIT can reverse Treg cell function and phenotype. CD4⁺ T cells purified from PBMCs of HDs were stimulated under specified conditions for 5 days and analyzed using flow cytometry. A Percentages of pAkt⁺ cells and mTOR ⁺ cells in Foxp3⁺Tregs. B Percentages of pSTAT1⁺ cells and STAT4⁺ cells in Foxp3⁺Tregs. C, D CD4⁺ T cells were pretreated using TIGIT agonists before exposing to TCR. C Percentages of pSTAT1⁺ cells, STAT4⁺ cells in Foxp3⁺Tregs. D Percentages of CXCR3⁺Foxp3⁺Th1-like Tregs. Data are mean ± SEM of seven independent experiments using different donors. *P < 0.05, **P < 0.01, **P < 0.001