Fig. 7

IFN-α mediated highly activated T cells following in-sequential effects in SLE. A PBMCs isolated from the peripheral blood of 16 SLE patients and 10 HDs shown in Supplementary Table S2 were analyzed using flow cytometry without incubation. Bar graphs showing the frequencies of CD69⁺ cells, PD-1⁺ cells, and CD25⁺ cells in CD4⁺ T cells. B Whole blood of patients with SLE was collected, stained directly or after the stimulation by IFN-α for 20 min. Bar graphs showing the MFI of pSTAT1 and pSTAT4 in Foxp3⁺Tregs analyzed using flow cytometry. C CD4⁺CD25⁺Tregs purified from ten PBMCs of SLE patients were stimulated under specified conditions for 5 days and analyzed using flow cytometry. Representative flow cytometry plots and bar graphs showing the frequencies of CXCR3⁺Foxp3⁺Th1-like Tregs in CD4⁺ T cells