Fig. 3
From: Prokineticin 2 is a catabolic regulator of osteoarthritic cartilage destruction in mouse
Prok2 modulates protein levels of MMP13 and type II collagen in cartilage explants. A Mouse cartilage explants, sourced from 4-day-old ICR mice, were treated with 1 × 109 pfu of Ad-C, Ad-HIF-2α, or Ad-Prok2 for 24 h. Presented are representative immunofluorescence images of type II collagen, MMP13, and DAPI in sections of cartilage explants (n=10 explants). B Mouse cartilage explants were treated with PBS as a control, 100 ng/ml of IL-6 as a positive control, and 100 ng/ml of rProk2 for 24 h. Representative immunofluorescence images of type II collagen, MMP13, and DAPI in sections of cartilage explants are presented (n=10 explants)