Fig. 6
From: Prokineticin 2 is a catabolic regulator of osteoarthritic cartilage destruction in mouse
Knockdown of Prok2 in joint tissues alone is insufficient to inhibit post-traumatic OA cartilage destruction. A Representative immunostaining images of Prok2 (left panel) and semi-quantification of Prok2-positeive pixels (right panel) in cartilage sections of mice received IA injection of 1 × 109 pfu of Ad-shC (control) or Ad-shProk2 (n=6 mice per group). B, C Sham and DMM-operated mice were subject to IA injections of 1 × 109 pfu of Ad-shC or Ad-shProk2 once a week for 3 weeks. Representative safranin-O staining images (B) and scoring of OARSI grade and osteophyte maturity (C, n=14 mice per group). D qRT-PCR analysis of the indicated molecules in primary-culture chondrocytes treated with 400 MOI of Ad-C and Ad-HIF-2α, with or without the specified MOI of Ad-sh-C and Ad-shProk2 (n=9). Values are presented as mean ± 95% CI, and significance was evaluated by paired t-test