Fig. 4

Resatorvid administration downregulates MyD88/NF-κB and NLRP3 signaling in the cartilage and synovium of CFA-induced TMJOA mice. A Representative immunofluorescence images analyzing the expression of MyD88 (red) and NF-κB (green) in the condylar cartilage of CFA-induced TMJOA mice at 2 and 4 weeks. B Quantitative analysis revealed that administration of Resatorvid reduced the rate of MyD88- and NF-κB-positive cells in the cartilage of CFA group mice at 2 and 4 weeks, respectively, one-way ANOVA, mean ± SEM, n = 6 (##p < 0.01, ###p < 0.001 vs saline group; *p < 0.05, **p < 0.01 vs CFA group). C, D Immunofluorescence staining detecting the production of MyD88 (red) and NF-κB (green) in the synovium of CFA-induced TMJOA mice at 4 weeks (C) and quantified by the average optical density of positive cells (D), one-way ANOVA, mean ± SEM, n = 6 (##p < 0.01, ###p < 0.001 vs saline group; *p < 0.05 vs CFA group). E, F Immunofluorescence double staining analyzing the coexpression of TLR4 (red) and NLRP3 (green) in the condylar cartilage of CFA-induced TMJOA mice at 4 weeks (E). The expression of TLR4 was significantly inhibited by Resatorvid administration, and the number of NLRP3-positive cells was also downregulated, as quantified by the rate of double-positive cells (F), one-way ANOVA, mean ± SEM, n = 5 (#p < 0.05 vs saline group; **p < 0.01 vs CFA group)