Fig. 5

FBP1 suppresses OA progression through promotion of the CRB3. A The Safranin O and fast green staining of cartilage of mice and immunohistochemistry staining for CRB3. Scale bars = 100 μm. B,C The quantitative analysis of the OARSI scale and CRB3-positive chondrocytes in OA mice. n = 5 per group. D,F Western blot and quantification of COLX, CRB3, SOX9, MMP13, P21, and P16 in siCRB3-treated primary chondrocytes from mice. E,G Western blot and quantification of CRB3, SOX9, MMP13, P21, P16, and FBP1 in oeFBP1-treated primary chondrocytes from mice. n = 5 per group.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS = not significant. One-way analysis of variance (ANOVA) was performed