Fig. 4

Joint immobilization induces sensory innervation in synovium in mice. A Representative images of H/E staining images in knee joints at 0, 2, 4, and 8 weeks after immobilization. Scale bar, 50 mm. B Representative images of immunofluorescence (IF) staining of CGRP staining (first row), DAPI staining (second row) and merged image (third row) in synovial tissue of knee joints at different groups. The white dashed lines indicate the synovial tissue. Scale bar, 50 mm. C Quantitative data of expression of CGRP based on staining results in B. N = 6 biologically independent replicates per group. Results were expressed as mean ± standard deviation (sd). **P < 0.01