Fig. 2
From: Wilforine inhibits rheumatoid arthritis pathology through the Wnt11/β-catenin signaling pathway axis
WFR inhibits the pathological-related genes and the FLS proliferation. ELISA showed that WFR effectively reduced TNF-α, IL-6, and IL-1β in serum of CIA rats (A). WFR effectively decreased the expression of MMP3 and fibronectin mRNA in the synovium of CIA rats (B). The cell dose of WFR was selected according to CCK8 experiment, and WFR significantly inhibited the proliferation of CIA FLS when the dose concentration was 200 nM (C). WFR effectively decreased the expression of MMP3 and fibronectin mRNA in CIA FLS (D). Immunofluorescence detection showed that WFR decreased the protein expression of MMP3 in CIA FLS (E) (scale bar = 50 μm). WB detection showed that WFR decreased the protein expression of fibronectin in CIA FLS (F). CCK-8 detection showed that WFR significantly inhibited the proliferation of CIA FLS (G). *CIA group vs normal, #CIA group+WFR vs CIA group, n = 3. *p < 0.01