Fig. 5
From: Wilforine inhibits rheumatoid arthritis pathology through the Wnt11/β-catenin signaling pathway axis
WFR inhibits the activation of Wnt/β-catenin signaling pathway. RT-qPCR results showed that WFR effectively decreased the expression of CCND1, GSK-3β, and c-Myc mRNA in the joint synovium of CIA rats (A), WFR effectively decreased the expression of CCND1, GSK-3β, and c-Myc mRNA in CIA FLS (B). WB detection showed that WFR decreased the protein expression of β-catenin (C), CCND1 (D), and c-Myc (E) in CIA FLS. Immunofluorescence assay showed that WFR reduced the expression of β-catenin into the nucleus in CIA FLS (F) (scale bar = 50 μm). *CIA group vs normal, #CIA group+WFR vs CIA group, n = 3. *p < 0.01