Fig. 6
From: Wilforine inhibits rheumatoid arthritis pathology through the Wnt11/β-catenin signaling pathway axis
Overexpression of Wnt11 reversed the effect of WFR. RT-qPCR results showed that Wnt11 mRNA expression significantly increased after Wnt11 overexpression (Wnt11-ove) (A). WB results showed that Wnt11-ove reversed the inhibitory effect of WFR on β-catenin protein expression in CIA FLS (B). RT-qPCR results showed that Wnt11-ove interfered with WFR to inhibit the mRNA expression of CCND1, GSK-3β, and c-Myc in CIA FLS (C). Wnt11-ove interfered with WFR inhibition of MMP3 and fibronectin mRNA expression in CIA FLS (D). CCK-8 results showed that Wnt11-ove interfered with the inhibitory effect of WFR on RA FLS (E). For A, *CIA FLS+Wnt11 ove vs CIA FLS. For B, C, D, E, *CIA FLS+WFR vs CIA FLS, #Wnt11 ove+WFR vs CIA FLS+WFR, n = 3. *p < 0.01