Table 1 Sequences of primers for RT-qPCR
From: Wilforine inhibits rheumatoid arthritis pathology through the Wnt11/β-catenin signaling pathway axis
Gene | Forward primer | Reverse primer |
|---|---|---|
Rat sequence | ||
β-catenin | ACAAGCCACAGGACTACAAGAAACG | TCAGCAGTCTCATTCCAAGCCATTG |
c-Myc | AGCAGCGACTCTGAAGAAGAACAAG | GGATGACCCTGACTCGGACCTC |
CCND1 | GAGGCGGATGAGAACAAGCAGATC | GGAGGGTGGGTTGGAAATGAACTTC |
MMP3 | CGTCGGTGGCTTCAGTACCTTTC | TCACCTCCTCCCAGACCTTCAAAG |
Fibronectin | AGGCACAAGGTCCGAGAAGAGG | GGTCAAAGCATGAGTCATCCGTAGG |
β-actin | CAACTGGCGGGACTTTCTCAAGG | CAGCACTGTGTTGGCATAGAGGTC |
GSK-3β | CAATCGCACTGTGTCGCCGTCTC | GGTGTGTCTCGCCCATTTGGTAG |
Wnt11 | CAGGATCCCAAGCCAATAAA | GACAGGTAGCGGGTCTTGAG |
Human sequence | ||
β-catenin | GGTGTCCAACACAGATCTGA | CCACTAGACCACTCATCTAC |
c-Myc | TTCCCCTACCCTCTCAACGAC | TTCTTCCTCATCTTCTTGTTCCTCC |
CCND1 | CTGTGCATCTACACCGACAACTA | AGGTTCCACTTGAGCTTGTTCAC |
MMP3 | TCCCAGGAAGATAGCTGAGG | CAACTGCGAAGATCCACTGA |
Fibronectin | AAGCTGCTGGAGCTGATAAGA | GTTACAGCCCAAACGACTGAC |
β-actin | TGTCACCAACTGGGACGATA | GGGGTGTTGAAGGTCTCAAA |
GSK-3β | ATGGGAAAATCAAAAGAAATCAGCC | CGCACCAAAGTACGTTCATCTCTA |
Wnt11 | AATCAGACGCAACACTGTAAAC | CTCGATGGAGGAGCAGTTC |