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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Exosome-mediated delivery of super-repressor IκBα alleviates inflammation and joint damages in rheumatoid arthritis

Fig. 1

Production and characterization of Exo-srIκB. A DNA constructs and blue-light-mediated fusion of recombinant proteins were used to produce Exo-srIκB, as shown in schematic diagrams. B Representative images for transmission electron microscopy of Exo-srIκB morphology. C Representative panels for concentration and size distribution of Exo-srIκB were determined by a NanoSight (NS300) instrument. D Immunoblotting of Expi293F cells (producing cells) and Expi293F cell-derived exosomes to analyze the expression of recombinant proteins (srIκB, CRY2, and CD9) and exosome markers (CD9, CD81, TSG101, Alix, and GAPDH) and cell organelle markers (GM130, lamin B1, prohibitin, and calnexin). Exo-Naïve (non-engineered cell and exosome) were used as negative controls of Exo-srIκB

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Arthritis Research & Therapy

ISSN: 1478-6362

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