Fig. 5

ADAM8 blockade suppresses FLS inflammation by inhibiting MAPK signaling cascades. A Western blot and quantitative analysis of TNF-α, IL-6 and COX2 after treatment with siADAM8. H qRT-PCR analysis of the mRNA expression levels of TNF-α, IL-6, COX2, and PGE2. D-F Immunofluorescence staining and quantitative analysis of TNF-α and IL-6 after treatment with siADAM8. G Western blot of P-JNK, JNK, P-ERK, ERK, P-P38, and P38 after treatment with siADAM8. H Quantitative analysis of P-JNK/JNK, P-ERK/ERK, and P-P38/P38. *P < 0.05; **P < 0.01