Table 1 List of genes and the primer details
Genes | Strand | Primer sequences | Size (bp) | AT (°C) | Origin |
|---|---|---|---|---|---|
Gapdh | A AS | 5′-TCACTGCCACCCAGAAGAC-3′ 5′-TGTAGGCCATGAGGTCCAC-3′ | 450 | 57.3 | Mouse |
Mmp3 | A AS | 5′-AGGGATGATGATGCTGGTATGG-3′ 5′-CCATGTTCTCCAACTGCAAAGG-3′ | 434 | 58.0 | Mouse |
Mmp10 | A AS | 5′-AGAAATGGACACTTGCACCCTCAG-3′ 5′-CTGTCCGTGTTGTGAGCCTCATAG-3′ | 448 | 61.5 | Mouse |
Mmp13 | A AS | 5′-TGATGGACCTTCTGGTCTTCTGG-3′ 5′-CATCCACATGGTTGGGAAGTTCT-3′ | 474 | 58.2 | Mouse |
Adamts4 | A AS | 5′-CATCCGAAACCCTGTCAACTT-3′ 5′-GCCCATCATCTTCCACAATAGC-3′ | 287 | 58.4 | Mouse |
Adamts5 | A AS | 5′-GCCATTGTAATAACCCTGCACC-3′ 5′-TCAGTCCCATCCGTAACCTTTG-3′ | 292 | 58.4 | Mouse |
Aggrecan | A AS | 5′-GAAGACGACATCACCATCCAG-3′ 5′-CTGTCTTTGTCACCCACACAT-3′ | 581 | 60.0 | Mouse |
Col2a1 | A AS | 5′-CACACTGGTAAGTGGGGCAAGACCG-3′ 5′-GGATTGTGTTGTTTCAGGGTTCGGG-3′ | 173 | 58.0 | Mouse |
Sox9 | A AS | 5′-ATGCTATCTTCAAGGCGCTG-3′ 5′-GACGTCGAAGGTCTCAATGT-3′ | 272 | 60.0 | Mouse |