Fig. 1
From: TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
The expression of TfR1 in cartilage of OA and primary chondrocytes. (A) Representative images of knee articular cartilage from control group and DMM group, showing TfR1-positive chondrocytes. Scale bars = 100 μm. The ratio of TfR1 positive cells and total chondrocytes in knee articular cartilage of control group and DMM group. (n = 8 for each group). (B) The expression of TfR1 in chondrocytes treated with 0-1-5-10ng/ml IL-1β for 12 h detected by western blotting. The density of the TfR1 immune-reactive bands was analyzed by using GAPDH expression as a loading control. (C) Representative images of immunofluorescence staining for TfR1 expression in chondrocytes treated with IL-1β for 12 h. Scale bars = 50 μm. (D) Representative images for ferrous ions in the indicated group and statistical analysis of fluorescence intensity (ferrous ions). Scale bars = 200 μm. Data are presented as the mean ± SD, * P < 0.05, **** P < 0.0001