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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Integrative analysis of single-cell and bulk transcriptome data reveal the significant role of macrophages in lupus nephritis

Fig. 5

Overexpression of LGALS9 elicits a significant pro-inflammatory effect on THP-1 cells. (A) The fluorogram of reporter GFP confirmed a successful lentivirus packaging and infection in THP-1 cells. (B, C) LGALS9 expression was significantly elevated in mRNA and protein levels in LGALS9_OE than in control THP-1_NC cells. The bar graph represents the mean and standard deviation of the results from three experiment replicates. (D) Heatmap of DEGs between LGALS9_OE cells and the negative control THP_1_NC cells. (E) Bubble plots of the results of GO enrichment analysis of the DEGs between LGALS9_OE and THP-1_NC cells. (F) Western blot of CD163 and CXCR3 in LGALS9_OE and THP-1_NC cells. (G) ELISA results of CCL3, CCL4 and CXCL8 in LGALS9_OE and THP-1_NC. The bar graph represents the mean and standard deviation of the results from three experiment replicates cells

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Arthritis Research & Therapy

ISSN: 1478-6362

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