Fig. 3
Investigation of the transcriptional regulatory function of the DMR in Tc28a2 chondrocytes. A Schematic representation of the promoter and enhancer plasmid vector constructs. B Lucia reporter assays assessing promoter or enhancer activity in the presence of construct containing the DMR in an unmethylated or methylated state. Values were normalised to those in empty vector control (dotted horizontal line). Black dots represent individual samples (12 replicates per group). Box plots show the median, 25th and 75th percentiles, and minimum and maximum values. P-values calculated by Mann Whitney U test with Holm-Å Ãdák correction. * = P < 0.05; *** = P < 0.001; **** = P < 0.0001