Fig. 4

Epigenetic modulation of the DMR in Tc28a2 chondrocytes. A Schematic representation of the 16 CpGs and of the five gRNAs (arrow pointing left, antisense strand; arrow pointing right, sense strand). cg26736200 (CpG8) and cg26661922 (CpG13) are highlighted. B Mean DNAm levels (%) of the 16 CpGs following expression of dCas9-DNMT3a protein in control (black), with non-targeting gRNA, or in samples with a targeting gRNA (coloured). Seven replicates for control and for each targeting gRNA. C Relative expression of the three WWP2 transcripts and of the two miR-140 strands following epigenetic editing with gRNAs. Values were normalised to non-targeting gRNA. P-values were calculated using a paired t-test with Benjamini-Hochberg correction. * = P < 0.05; ** = P < 0.01; ns = not significant (P > 0.05)