Correction: mPGES-1 null mice are resistant to bleomycin-induced skin fibrosis

  • Matthew R McCann1,

    Affiliated with

    • Roxana Monemdjou2,

      Affiliated with

      • Parisa Ghassemi-Kakroodi1,

        Affiliated with

        • Hassan Fahmi1,

          Affiliated with

          • Gemma Perez2,

            Affiliated with

            • Shangxi Liu1,

              Affiliated with

              • Xu Shi-wen3,

                Affiliated with

                • Sunil K Parapuram1,

                  Affiliated with

                  • Fumiaki Kojima4,

                    Affiliated with

                    • Christopher P Denton3,

                      Affiliated with

                      • David J Abraham3,

                        Affiliated with

                        • Johanne Martel-Pelletier2,

                          Affiliated with

                          • Leslie J Crofford4,

                            Affiliated with

                            • Andrew Leask1 and

                              Affiliated with

                              • Mohit Kapoor2Email author

                                Affiliated with

                                Arthritis Research & Therapy201113:402

                                DOI: 10.1186/ar3285

                                Published: 24 March 2011

                                Correction

                                Following publication of our recent article [1], an error in Figure 5d was noticed. In Figure 5d, the β-actin blot corresponding to α-SMA blot was captured on the chemiluminescence imaging system in an inverted orientation. Since this imager does not capture the protein marker, an error in the orientation of the membrane while capturing the picture of the blot occurred. During preparation of the figures for Figure 5d, the α-SMA blot was in right orientation but since orientation of β-actin blot was inverse as it was captured inversely resulted in non-corresponding β-actin blot with respect to α-SMA blot. We have rectified this error now and β-actin blot now corresponds to α-SMA blot. The corrected figure 5 is given here as Figure 1 and a supplementary figure 1 (Additional file 1) showing another set of blots representing Figure 5d is also provided.
                                http://static-content.springer.com/image/art%3A10.1186%2Far3285/MediaObjects/13075_2011_3034_Fig1_HTML.jpg
                                Figure 1

                                mPGES-1 genetic deletion results in reduced collagen content and myofibroblast formation in vivo. (a) Hydroxyproline analysis showed reduced collagen content in mPGES-1 null mice compared with wild-type (WT) mice in response to bleomycin treatment. Data from four separate mice per group are shown. (b, c) Immunohistochemistry using anti-α-SMA antibody was performed. mPGES-1 null mice showed a reduced number of α-SMA-expressing myofibroblasts compared with WT mice in response to bleomycin treatment (4-week treatment). Representative data from four separate animals per group are shown. *P < 0.05; bleomycin-treated WT and mPGES-1 null mice compared with phosphate-buffered saline (PBS)-treated mice. + P < 0.05; bleomycin-treated mPGES-1 null mice compared with bleomycin-treated WT mice. (d) Protein extracts from skin tissue after 4 weeks of bleomycin or PBS treatment were subjected to Western blot analysis with an anti-α-SMA antibody. mPGES-1 null mice treated with bleomycin showed reduced α-SMA expression compared with bleomycin-treated WT mice. Representative blot from four separate animals per group is shown. α-SMA, alpha-smooth muscle actin; mPGES-1, microsomal prostaglandin E2 synthase-1.

                                Declarations

                                Authors’ Affiliations

                                (1)
                                The Canadian Institute of Health Research Group in Skeletal Development and Remodeling, Division of Oral Biology and Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
                                (2)
                                Osteoarthritis Research Unit, University of Montreal Hospital Research Center (CR-CHUM) and Department of Medicine, University of Montreal
                                (3)
                                Centre for Rheumatology, Department of Medicine, University College London (Royal Free Campus)
                                (4)
                                Division of Rheumatology, Department of Internal Medicine, University of Kentucky

                                References

                                1. McCann MR, Monemdjou R, Fahmi H, Perez G, Liu S, Shi-wen X, Parapuram SK, Kojima F, Denton CP, Abraham DJ, Martel-Pelletier J, Crofford LJ, Leask A, Kapoor M: mPGES-1 null mice are resistant to bleomycin-induced skin fibrosis. Arthritis Res Ther 2011, 13:R6.PubMedView Article

                                Copyright

                                © BioMed Central Ltd 2011

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