Volume 14 Supplement 1

Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Open Access

GCIP, Id like HLH protein, negatively regulates cell proliferation of rheumatoid synovial cells via interaction with CBP

  • Hidetoshi Fujita1, 2,
  • Minako Nakazawa1,
  • Satoko Aratani1, 3,
  • Kusuki Nishioka3,
  • Akiyoshi Fukamizu4 and
  • Toshihiro Nakajima1, 3
Arthritis Research & Therapy201214(Suppl 1):P17

DOI: 10.1186/ar3618

Published: 29 February 2012


Rheumatoid arthritis (RA) is one of the most common articular diseases with a prevalence of 1% worldwide [1, 2]. The clinical features of RA include chronic inflammation of systemic joints associated with synovial hyperplasia followed by impairment of quality of life [3, 4]. Recently, we have shown that Synoviolin/Hrd1, an E3 ubiquitin ligase, is a novel causative factor for arthropathy [5]. However, the mechanism that regulates synovial cell outgrowth is not fully understood.

Materials and methods

Human embryonic kidney (HEK)-293 cells, HEK-293T cells, NIH3T3 cells and synovial cells were cultured in DMEM medium. Transient transfection assays were performed in HEK-293 cells and HEK-293T cells. HEK-293 cells transfected with NF-κB-Luc were treated with 100 ng/ml of phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), or 10 ng/ml of TNF-α for 24 h, and luciferase activities were measured. siRNAs with 21 nucleotides for human GCIP were chemically synthesized. Transfection with siRNAs and cell survival assay were carried out.


Grap2 cyclin D interacting protein (GCIP), Id like HLH protein, was down-regulated in the rheumatoid synovial cells. Introduction of GCIP into mouse fibroblast NIH3T3 cells resulted in growth suppression, whereas knockdown with siRNAs in synovial cells enhanced cell growth. GCIP associated with CBP and repressed transcription of CREB-target genes such as cyclin D1 by inhibition of interaction between CBP and RNA polymerase II complexes. Binding assays revealed that GCIP bound to CBP via acidic region, not HLH domain, and this interaction was regulated by phosphorylation of GCIP in a cell cycle-dependent manner. Therefore, GCIP has inhibitory effect on cell proliferation via interference with CBP-mediated transcription.


We propose the novel inhibitory mechanisms of Id protein family; the coactivator CBP is a functional target. Furthermore, down-regulation of GCIP may be a key factor in rheumatoid synovial cell outgrowth.

Authors’ Affiliations

Institute of Medical Science, St Marianna University School of Medicine
Advanced Radiation Biology Research Program, and Hospital, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
Institute of Medical Science, Tokyo Medical University
Graduate School of Life and Environmental Sciences, University of Tsukuba


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© Fujita et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.