Figure 4

Production of antibody (Ab) specific for murine type II collagen (mCII) by double DR1-T cell receptor (TCR) Tg and DR1 Tg mice. Double- and single-transgenic littermates were immunized with 100 μg of bCII emulsified in CFA (Complete Freund’s Adjuvant) as described in Methods. Serum samples were collected 27 days after immunization and analyzed for quantity of mCII-specific Ab by ELISA. Units of Ab were calculated using standard reference sera. Data are expressed as mean titers of 10 mice per group ± standard error of the mean (total IgG titers, double-transgenic versus single-transgenic, 180.5 ± 34 versus 50.2 ± 11, P = 0.001; IgG1 titers, double-transgenic versus single-transgenic: 61.5 ± 22.3 versus 12.3 ± 6.1, P = 0.007; IgG2b titers 143.45.9 versus 51.3 ± 16.6, P = 0.0008; IgG3 titers 13.1 ± 5.6 versus 14.2 ± 3.2, P = 0.69).