Characterization of T cell phenotype and function in a double transgenic (collagen-specific TCR/HLA-DR1) humanized model of arthritis
© Tang et al.; licensee BioMed Central Ltd. 2014
Received: 1 April 2013
Accepted: 27 December 2013
Published: 10 January 2014
T cells orchestrate joint inflammation in rheumatoid arthritis (RA), yet they are difficult to study due to the small numbers of antigen-specific cells. The goal of this study was to characterize a new humanized model of autoimmune arthritis and to describe the phenotypic and functional changes that occur in autoimmune T cells following the induction of pathological events.
We developed a double transgenic mouse containing both the HLA-DR1 transgene and an HLA-DR1-restricted collagen-specific TCR in order to obtain large numbers of antigen-specific T cells that can be used for immunologic studies.
In vitro, CII-specific T cells from this mouse proliferated vigorously in response to the CII immunodominant peptide A2 and the cells altered their phenotype to become predominately CD62Llow and CD44high “activated” T cells. The response was accompanied by the production of Th1, Th2, and Th17-type cytokines. Following immunization with bovine CII/CFA, these mice develop an accelerated arthritis compared to single transgenic HLA-DR1 mice. On the other hand, when the mice were treated orally with the analog peptide A12, (a suppressive analog of collagen we have previously described), arthritis was significantly suppressed, despite the fact that >90% of the CD4+ T cells express the TCR Tg. In GALT tissues taken from the A12-treated mice, IL-2, IFN-γ, and IL-17 production to the autoimmune collagen determinant dropped while high levels of IL-10 and IL-4 were produced.
We have developed a humanized model of autoimmune arthritis that will be useful for the study of T cell directed therapies as well as T cell mediated mechanisms of autoimmune diseases.
Although the majority of autoreactive T cells are deleted in the thymus, it is clear that some escape thymic selection, and once in the periphery are capable of mediating an autoimmune response. However, the precise mechanism by which T cell function is altered so that autoimmune diseases can develop is poorly understood. Some of the difficulty in deciphering these mechanisms is that the frequency of the Ag-specific T cells to be targeted is very limited, estimated to be 104 or less . Here, we describe the development of a double-transgenic mouse that expresses an autoreactive T cell receptor (TCR) specific for type II collagen (CII) and the human leukocyte antigen (HLA) -DRB1*0101, a haplotype susceptible to the induction of collagen-induced arthritis. These mice express a TCR specific for the HLA-DR1-restricted immunodominant determinant of CII, A2, on >90% of the CD4+ T cells. Despite the fact that they are capable of recognizing murine CII (mCII), they are not deleted in the thymus.
We have analyzed the function of these T cells using the collagen-induced arthritis model with the goal of determining the role that autoreactive T cells play in mediating autoimmune arthritis and in modulating the severity of the disease. We also investigated whether these autoreactive transgenic T cells could be manipulated to secrete primarily suppressive cytokines, thereby downregulating autoimmunity. We have treated these mice orally with an altered peptide ligand (APL) of collagen to determine how this therapy influences the cytokine responses of T cells located in murine gut-associated lymphatic tissue (GALT). We believe this transgenic (Tg) model can serve as an excellent vehicle for defining the molecular mechanisms by which T cells influence autoimmune arthritis and will help shape the development of future T-cell directed therapies for autoimmune diseases .
Preparation of tissue-derived CII and synthetic peptides
The following nomenclature is used to define the antigens used in this study: type II collagen, CII; bovine type II collagen, bCII; murine type II collagen, mCII; a peptide containing the immunodominant determinant sequence of both bovine and human CII (GIAGFKGEQGPKGEB), A2; a peptide containing the immunodominant determinant sequence of murine CII (GIAGFKGDQGPKGEB), mA2; a synthetic peptide representing the sequence (GIAGNKGDQGPKGEB), A12; the constituent protein chains of bovine CII isolated by carboxymethyl-cellulose chromatography, α1(II). B stands for 4-hydroxyproline.
Native type II collagen (CII) was solubilized from fetal calf articular cartilage or murine articular cartilage by limited pepsin-digestion and purified as described earlier . The purified collagen was dissolved in cold 10 mM acetic acid at 4 mg/ml and stored frozen at -70°C until used. In some experiments denatured α1(II) chains were used. The synthetic peptides were supplied by Biomolecules Midwest Inc. (Waterloo, IL, USA).
Generation of Tg mice expressing DR
Mice expressing the chimeric (human/mouse) DRB1*0101 construct were maintained in our onsite facility. The chimeric DRB1*0101 construct and the production of Tg mice expressing this construct have been previously described .
Generation of double-transgenic (DR1-TCR) mice
The CII-specific TCR-genes in the DR-transgenic mice, were expressed using the cassette vectors pTαcass and pTβcass provided by Benoist and Mathis . Rearranged Vα2/Jα27 and Vβ8.1/D/Jβ2.4 fragments were obtained from a DR1-restricted A2-specific T cell hybridoma (E168) by rt-PCR. The PCR fragments were subcloned into the TCR cassette vectors, using the following modifications. For the α chain construct, a Xma I site was created 17 bp upstream of the ATG cordon of the TCR-Vα2/Jα27 cDNA; and an 11-bp sequence that represents the beginning of the J-C intron was added downstream of the Jα27 segment, followed by an artificial Not I site. The resulting cDNA fragment was introduced into the pTαcass between the Xma I and Not I sites to create the pTα cass-Vα2. For the β chain construct, a Xho I site was created 12 bp upstream of the ATG cordon of Vβ8.1, and a 17 bp untranslated sequence representing the beginning of the J-C intron was introduced downstream of Jβ2.4, followed by a Sac II site. The resulting cDNA was introduced into the cassette vector pTβcass between the Xho I and Sac II sites to create pTβcass-Vβ8.1. The linearized DNA fragments (pTαcass-Vα2 and pTβcass-Vβ8.1) were simultaneously injected into fertilized eggs of FVB/N mice. Transgenic founders were identified by PCR analysis of tail DNA. Mice were backcrossed onto DR1-transgenic C57BL/10 mice to establish a TCR transgenic mouse line, and then backcrossed to the HLA-DR1 mice to develop double-transgenic mice. Mice used in these experiments were from generations N13 to N14 and were heterozygous for the TCR transgenes and homozygous for the HLA-DR1 transgene.
All mice were fed standard rodent chow (Ralston Purina Co., St Louis, MO, USA) and water ad libitum. Sentinel mice were routinely tested for murine pathogens. Experiments were conducted in accordance with approved Institutional Animal Care and Use Committee (IACUC) protocols. Mice were 8 to 12 weeks old.
The antibodies (Abs) used for flow cytometry included: peridinin chlorophyll protein (PerCP)-conjugated anti-CD4 (clone RM4-5), phycoerythrin (PE)-conjugated anti-CD8 (clone 53–6.7), PE-conjugated anti-CD62L (clone Mel-14), fluorescein isothiocyanate (FITC)-conjugated anti-V β8.1,2 (clone F23.1), APC-conjugated anti-CD44 (clone IM7), and FITC-conjugated anti-Vα2 (clone: B20.1). All Abs were purchased from BD PharMingen (San Diego, CA, USA) and used according to the manufacturer’s recommendations.
Splenocytes, peripheral blood cells, or inguinal lymph node cells from mice were isolated and the phenotype was determined by multiparameter flow cytometry using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA). Cells were labeled with fluorochrome antibodies specific for CD4, CD25, or CD44, (BD Biosciences). In some experiments intracellular labeling was performed using antibodies specific for FoxP3 (FJK-16 s, eBioscience, San Diego, CA, USA). In the experiments involving intracellular staining, gating was performed on both CD4+ and Vβ8+ cells. A minimum of 10,000 cells was analyzed from each sample and the final analysis was performed using FlowJo software (Tree Star, Ashland, OR, USA).
Spleen cells were cultured with various concentrations of collagen and collagen peptides in 96-well plates at 4.5 × 105/well in 300 μl of HL-1 medium supplemented with 50 μM of 2-ME and 0.1% BSA (fraction V, Gig free, low end toxin; Sigma-Aldrich, St Louis, MO, USA) at 37°C, 5% humidified CO2 for 4 days. Eighteen hours before the termination of the cultures, 1 μCi of (3H)thymidine (New England Nuclear, Boston, MA, USA) was added to each well. Cells were harvested onto glass-fiber filters and counted on a Matrix 96 direct ionization beta counter (Packard Instrument, Meriden, CT, USA). Results were confirmed by replicate experiments and all data are expressed as disintegrations per minute (dpm).
Immunizations and arthritis induction
Mice were immunized with bCII for the induction of arthritis. bCII was dissolved in 10 mM cold acetic acid. The bCII was then emulsified with CFA (Complete Freund’s Adjuvant) as previously described . Mice were immunized subcutaneously at the base of the tail with 100 μg of bCII. For other immunizations, either peptide A12 or Ova emulsified with CFA was given subcutaneously so that each mouse received 100 μg of protein.
Measurement of the severity of arthritis
The severity of arthritis was determined by visually examining each forepaw and hindpaw and scoring them on a scale of 0 to 4 as described previously . Scoring was conducted by two examiners, one of whom was unaware of the identity of the treatment groups. Each mouse was scored thrice weekly beginning 3 weeks post immunization and continuing for 8 weeks. The mean severity score (sum of the severity scores for the group on each day/total number of animals in the group) was recorded at each time point.
Measurement of serum antibody titers
Mice were bled at 4 weeks after immunization and sera were analyzed for antibodies reactive with native murine CII using a previously described modification of an ELISA . Serial dilutions of a standard serum were added to each plate. From these values, a standard curve was derived by computer analysis using a four-parameter logistic curve. Results are reported as units of activity, derived by comparison of test sera with the curve derived from the standard serum which was arbitrarily defined as having 50 units of activity. Reactivity to mCII was not detected in sera obtained from normal mice.
Adoptive transfer experiments
Inguinal lymph-node cells from DR1-TCR double-transgenic mice previously immunized with either A12 or Ova in CFA were collected 8 days after immunization and the CD4+ cell subset was fractionated using ferromagnetic beads (Miltenyi Biotec, Gladbach, Germany) according to the manufacturer's protocol. The purity of the resulting cell population was confirmed by flow cytometry to have >95% purity. Recipient mice were given cells intravenously and received 5 × 105 cells. All recipient mice were immunized with bCII on the day of the cell transfer and observed for arthritis.
Oral treatment with peptide
For the study of events following oral administration of the peptide A12 in the DR1-TCR Tg mice, either A12 or Ova was dissolved in PBS. DR1-TCR mice were fed doses of 10, 50, or 100 μg A12, or 50 μg Ova by oral gavages three times a week. The treatments began either the day after immunization of mice with bCII/CFA (prevention protocol) or the day of the onset of arthritis (treatment protocol) and continued for the duration of the experiment.
Measurement of cytokines
To measure cytokines, in some experiments, naïve splenocytes from DR1-TCR transgenic mice were cultured with bovine α1(II), A2, mA2, or A12. In other experiments, DR1-TCR transgenic and DR1 Tg mice were immunized with bCII/CFA and lymph-node cells were taken from the mice 10 days after immunization and were cultured with a peptide representing the immunodominant determinant of murine collagen (mA2) so that supernatants could be analyzed for the presence of cytokines. In a third set of experiments, DR1-TCR double-transgenic mice were treated with 8 doses of 50 μg of either A12 or PBS by oral gavage, sacrificed after the last gavage and GALT cell populations were removed. Single-cell suspensions of mesenteric lymph-node cells and Peyer’s patch cells were made and pooled prior to culture in 48-well plates in triplicates. Each well contained 500 μl single cell suspension (5 × 105/ml) + indicated concentrations of each peptide, or PBS. Media used were X-VIVO 10 (Biowhittaker, Radnor, PA) for two-day cultures or DMEM-complete (DMEM + 1% Pen-strep, 1% 2-ME, 1% glutamax, 1% sodium pyruvate, 10% FBS) for 6-day cultures. Cells were incubated at 37°C and harvested after 72 hours.
Each culture was set up using cells from three mice run in triplicates and supernatants were analyzed for the presence of IL-10, IL-4, IFN-γ, and IL-17A using a Bio-plex mouse cytokine assay (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s protocol. Values are expressed as picograms per ml and represent the mean values for each group taken from three separate experiments.
Mean severity scores, antibody titers, and cytokine levels were compared using the Mann–Whitney test. The numbers of arthritic limbs were compared using Fischer’s exact test.
Generation of the double-transgenic mice
Phenotype of TCR T cells
Cytokines produced in response to murine collagen
No Ag (antigen)
17 ± 5
mA2 (3 μmol/ml)
13,522 ± 154
9,849 ± 255
297 ± 24
mA2 (0.3 μmol/ml)
5,167 ± 95
5,206 ± 175
142 ± 19
No Ag (antigen)
59 ± 11
184 ± 43
23 ± 12
mA2 (3 μmol/ml)
1,116 ± 22*
1,082 ± 155*
85 ± 18*
mA2 (0.3 μmol/ml)
737 ± 38*
695 ± 29*
42 ± 11*
Similarly, when α1(II) was administered in vivo to the double transgenic mice, the CD4+ T cells tested directly ex vivo were found to have increased numbers of activated CD44hi and CD62Llo CD4+ cells (Figure 3B). On the other hand, the addition of the A12 peptide significantly decreased the expression of activation markers. Staining for intracellular Foxp3 did not reveal differences between the two groups tested directly ex vivo (Figure 3B).
Induction of autoantibodies and disease in DR1-TCR Tg mice
Induction of suppression of arthritis
Treatment of arthritis
Our priority, however, was to establish whether arthritis could be suppressed in the double-transgenic mice once the immune response had been established. We elected to use the oral route of administration, a route more easily translatable into human usage. Therefore, when double-transgenic mice were treated orally with A12 in a preventive protocol (Figure 6, panel B), we found that A12 significantly reduced the severity of arthritis in the double-Tg mice. The mice treated with OVA developed arthritis at an expected accelerated rate, whereas by the A12-treated mice had significantly less severe disease. Interestingly, the dose response exhibited a bell-shaped curve with the 50 μg dose having the greatest effect on arthritis with protection afforded for the duration of the treatment. Mice treated with ether 10 μg or 100 μg had less suppression (Figure 6, panel B). In another set of experiments, mice were treated with the A12 peptide using a treatment protocol. Mice were fed daily doses of either PBS or 50 μg if A12, beginning the day of the onset of arthritis (Figure 6, panel C). Again, oral treatment with A12 significantly attenuated the severity of arthritis.
Cytokine responses from mice treated with oral A12
10 ± 7
17 ± 8
17 ± 10
5 ± 3
280 ± 22
256 ± 28
45 ± 14
25 ± 6
2 ± 3
2 ± 1
15 ± 9
4 ± 3
12 ± 20*
2 ± 2*
105 ± 18*
48 ± 8*
One of the key roles of the immune system is to protect the organism against infectious diseases and cancer, while avoiding autoimmune damage. Although T cell responses are expected to be tightly regulated to prevent T cell hyperactivation, which may lead to autoimmune pathology, our data clearly demonstrate that autoreactive T cells can be activated and induced to secrete inflammatory cytokines, causing significant tissue damage, and playing a major role in increasing the severity of autoimmune arthritis. Although others have previously observed that T cells play a critical role in autoimmunity in other animal models, that is, proteoglycan (PG)-induced arthritis (PGIA) and K/BxN spontaneous arthritis  as well as collagen-induced arthritis (CIA) , the ability to make double-transgenic mice that have a large number of T cells restricted by the common RA-susceptible major histocompatibility complex (MHC) molecule, DRB*0101 (DR1), allows greater ease and clarity in investigating the mechanisms by which T cells become dysregulated during the initiation of arthritis or how their behavior is altered when exposed to new therapies.
We demonstrated that in the CIA autoimmune arthritis model, immunization with bCII/CFA causes an accelerated and more severe arthritis compared to mice bearing only the HLA-DR1 transgene. In vitro, bCII-specific double-transgenic T cells readily demonstrate both proliferation and production of Th1, Th2, and Th17-type cytokines when challenged with the A2 peptide, reflecting the presence of a large number of fully functional CII-reactive T cells. Using this model, we have confirmed that activation of a large number of DR1-restricted autoimmune CD4+ T cells enhances the severity of the arthritis, and this phenomenon is associated with both increased numbers of autoantibodies and enhanced levels of inflammatory cytokines. These data reflect the concept that T-cell recognition of a systemically expressed self-peptide can lead to more severe disease, and that inflammatory CD4 + T-cells are dysregulated in autoimmune arthritis [12–15].
On the other hand, an understanding of the mechanisms by which T cells can be manipulated to control disease is critical if T cell-specific therapies are to be successfully implemented. CD4+ helper T cells differentiate into specialized subsets, which attain restricted patterns of cytokine secretion and specific expression of master transcription factors in response to pathogens . However, some of the distinctions between these groups are beginning to blur. Therefore, it becomes imperative to clearly define the functional and phenotypic attributes of autoreactive cells during the induction of suppression.
We have used the new humanized double-transgenic model of arthritis to test oral administration of the altered peptide ligand A12 peptide and to demonstrate that T cells found in the GALT tissues release IL-10 and IL-4 in response to a murine autoantigen . Our therapy is based on our previous discovery that A12 could profoundly suppress arthritis in the CIA model by inducing a unique inhibitory T cell . When the A12 peptide was administered orally to double-transgenic mice, arthritis was significantly suppressed, despite the fact that >90% of the CD4+ T cells express the Tg. The IL-2, IFN-γ, and IL-17 production dropped and was replaced by IL-10 and IL-4. The response to A12 was dose dependent, with low doses being more effective than high doses. We believe that the bell-shaped curve induced by oral A12, is consistent with antigen processing by the GALT, leading to T cell cytokine secretion. Other investigators have reported that proteins administered orally in low doses require the induction of both IL-4 and IL-10 to suppress disease and that this induction is consistent with processing by the GALT. High doses of antigens involve a different mechanism [19, 20]. Our finding that A12 stimulates the secretion of IL-4 and IL-10 in GALT tissues, leading to suppression of arthritis, is consistent with evidence from other animal models, showing that release of IL-10 and IL-4  inhibits inflammatory bowel disease , experimental autoimmune encephalomyelitis , and diabetes .
The success of the best-known altered peptide ligand (APL), Copaxone (glatiramer acetate) as a T cell-directed first-line therapy for multiple sclerosis (MS), suggests that peptide therapies based on naturally occurring proteins may provide an effective first-line alternative to biologic drugs for the treatment of arthritis and that animal models play a critical role in determining their efficacy and safety profiles. Although earlier clinical trials of glatiramer had conflicting results, two more recent trials have demonstrated its efficacy in treating MS, that is, the phase-3, randomized CONFIRM trial [23–26] and a large National Institutes of Health (NIH)-sponsored (CombiRx Trial) . Its safety profile suggests that drugs based on APLs will have an excellent risk/benefit ratio .
We believe that this double-transgenic animal model of arthritis will be useful for the development and refinement of increasingly more sophisticated T cell-directed therapies for RA [29, 30]. The difficulty of obtaining human GALT suggests that murine models will be invaluable in precisely delineating the role that T cells play when therapies are administered orally.
This study provides a detailed description of the phenotype of a new double-transgenic (DR1, CII-specific TCR) mouse. We believe that this mouse will become an important tool to allow careful dissection of the role that T cells play in regulating autoimmune arthritis, and may shape new therapeutic approaches to autoimmune diseases. As several strategies for enhancing the efficacy of adoptive T cell therapy have been developed and introduced in clinical settings , we believe that this humanized arthritis model will be invaluable in advancing our understanding of the mechanisms that control the pathogenesis of RA.
synthetic peptide representing the sequence (GIAGNKGDQGPKGEB)
peptide containing the immunodominant determinant sequence of bovine type II collagen (GIAGFKGEQGPKGEB)
altered peptide ligand
bovine type II collagen
bovine serum albumin
type II collagen
Dulbecco's modified Eagle's medium
disintegrations per minute
enzyme-linked immunosorbent assay
fetal bovine serum
gut-associated lymphatic tissue
hematoxylin and eosin
human leukocyte antigen
murine type II collagen
major histocompatibility complex
- murine A2:
peptide containing the immunodominant determinant sequence of murine type II collagen (GIAGFKGDQGPKGEB)
peridinin chlorophyll protein
T cell receptor
constituent protein chains of bovine type II collagen isolated by carboxymethyl-cellulose chromatography.
This work was supported in part by USPHS Grants AR-55661, AR-55266, and program-directed funds from the Department of Veterans Affairs and the Arthritis Foundation.
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