Mouse | Culture | | Cytokines (pg/ml) | |
|---|
| | | IFN-γ | IL-17 | IL-4 |
|---|
DR1/TCRtg | No Ag (antigen) | 0 | 17 ± 5 | 0 |
| | mA2 (3 μmol/ml) | 13,522 ± 154 | 9,849 ± 255 | 297 ± 24 |
| | mA2 (0.3 μmol/ml) | 5,167 ± 95 | 5,206 ± 175 | 142 ± 19 |
DR1 | No Ag (antigen) | 59 ± 11 | 184 ± 43 | 23 ± 12 |
| | mA2 (3 μmol/ml) | 1,116 ± 22* | 1,082 ± 155* | 85 ± 18* |
mA2 (0.3 μmol/ml) | 737 ± 38* | 695 ± 29* | 42 ± 11* |
- Inguinal lymph-node cells from type II collagen (CII)-immunized DR1 or DR1-T cell receptor (TCR) Tg mice were taken from the mice 10 days after immunization and were cultured with peptide mA2 so that supernatants could be analyzed for the presence of cytokines. The numbers indicated represent the means ± standard error of the mean of three separate experiments. When cultures from DR1/TCR tg mice were compared with those from DR1 mice, the cytokine responses to collagen were all elevated, T helper (Th)1, Th2, and Th17. *P ≤0.01 when mA2 responses from DR1 mice were compared with mA2 responses from DR1-TCR/tg mice. Ag, antigen; mA2, murine A2 peptide.
