- Meeting abstract
Cytokine imbalance in NZB/W mice is associated with autoantibody levels and nephritis
Arthritis Res Ther volume 6, Article number: 23 (2004)
In the NZB/W murine model of lupus, various studies have analyzed cytokine production during disease development with contradictory results.
The aim of this study was to analyze cytokine pattern of splenic T cells from NZB/W mice. We assessed the possibility that a shift in cytokine production is associated with age, disease activity, or manifestation of nephritis.
NZB/W mice of different ages spanning 5–36 weeks were analyzed, and healthy (BALB/c × NZW) F1 mice were used as controls. Proteinuria was measured using albustix. Plasma creatinine and autoantibody levels were detected by commercial test kid and by ELISA, respectively. Splenic CD4+ T cells stimulated with PMA/Ionomycin were analyzed for intracellular cytokine staining by FACS analysis.
The increased frequency of IFN-γ producing CD4+ T cells correlated with age, anti-dsDNA IgG antibody levels and proteinuria. The increasing number of IL-10 producers was only associated with anti-dsDNA antibody levels and proteinuria. A small gain in IL-4+ T cells correlated with plasma creatinine. Neither the number of IL-10 nor that of IL-4 exhibiting T cells correlated with age. There was no significant change observed in the production of TNF-α. Calculating the IFN-γ/IL-4 ratio, an increasing shift toward a Th1 response was observed. The shift strongly correlated with anti-dsDNA antibody titres and proteinuria. In contrast, no cytokine shift could be observed in control mice with increasing age.
About this article
Cite this article
Enghard, P., Langnickel, D., Hiepe, F. et al. Cytokine imbalance in NZB/W mice is associated with autoantibody levels and nephritis. Arthritis Res Ther 6 (Suppl 1), 23 (2004). https://doi.org/10.1186/ar1065