Skip to main content

Tolerogenic dendritic cells differentially modulate naïve and memory CD4+ T cells

Background

Dendritic cells (DC) can be alternatively activated to induce tolerogenic DC, making them a promising therapy for autoimmunity. Because naïve and memory T cells have different requirements for tolerisation, we tested the modulatory activity of tolerogenic DC on both subsets of T cells.

Methods

Human monocyte-derived DC were matured with lipopolysaccharide (LPS-DC) or treated with dexamethasone, vitamin D3 and lipopolysaccharide (LPS-DexD3 DC) to obtain tolerogenic DC. DC were cocultured with allogeneic naïve or memory CD4+ T cells. Primed T cells were rested and restimulated with LPS-DC or CD3/CD28 beads.

Results and conclusions

LPS-DexD3 DC have reduced stimulatory capacity for both naïve and memory T cells. However, restimulation of T cells revealed a distinct difference between naïve and memory T cells that had been primed by LPS-DexD3 DC. Naïve T cells did not become anergic but were skewed to a regulatory phenotype (low IFNγ and high IL-10 production), whereas memory T cells were rendered hyporesponsive, with low proliferation and cytokine production. Thus, naïve and memory T cells are differently regulated by LPS-DexD3 DC. These data have implications for the use of tolerogenic DC vaccines as immunomodulators.

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Anderson, A.E., Sayers, B., Diboll, J. et al. Tolerogenic dendritic cells differentially modulate naïve and memory CD4+ T cells. Arthritis Res Ther 9, P10 (2007). https://doi.org/10.1186/ar2236

Download citation

Keywords

  • Public Health
  • Dendritic Cell
  • Dexamethasone
  • Cytokine Production
  • Modulative Activity