Table 1 Sequences of primers used in the real-time reverse transcription-polymerase chain reaction
From: Increased lymphangiogenesis in joints of mice with inflammatory arthritis
Genes | Sequences of primers | Accession number | Target sites | Size (base pairs) |
|---|---|---|---|---|
VEGF-A | F: 5'-TTTACTGCTGTACCTCCACCA-3' | M95200 | 125–423 | 298 |
| Â | R: 5'-ATCTCTCCTATGTGCTGGCTTT-3' | Â | Â | Â |
VEGF-B | F: 5'-CCTGGAAGAACACAGCCAAT-3' | NM_011697 | 537–701 | 165 |
| Â | R: 5'-GGAGTGGGATGGATGATGTC-3' | Â | Â | Â |
VEGF-C | F: 5'-GGGAAGAAGTTCCACCATCA-3' | NM_009506 | 1258–1392 | 135 |
| Â | R: 5'-ATGTGGCCTTTTCCAATACG-3' | Â | Â | Â |
VEGF-D | F: 5'-GCTGTCACTGTTGCCCACTA-3' | NM_010216 | 1423–1611 | 207 |
| Â | R: 5'-CCCTTCCTTTCTGAGTGCTG-3' | Â | Â | Â |
PLGF | F: 5'-GGGAAGAAGCAAGACATGGA-3' | NM_008827 | 1055–1261 | 189 |
| Â | R: 5'-ATGTCCTGTCCCATCTCCAG-3' | Â | Â | Â |
β-actin | F: 5'-ACCCAGATCATGTTTGAGAC-3' | X03765 | 280–503 | 224 |
| Â | R: 5'-GTCAGGATCTTCATGAGGTAGT-3' | Â | Â | Â |