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Table 1 Sequences of primers used in the real-time reverse transcription-polymerase chain reaction

From: Increased lymphangiogenesis in joints of mice with inflammatory arthritis

Genes Sequences of primers Accession number Target sites Size (base pairs)
VEGF-A F: 5'-TTTACTGCTGTACCTCCACCA-3' M95200 125–423 298
  R: 5'-ATCTCTCCTATGTGCTGGCTTT-3'    
VEGF-B F: 5'-CCTGGAAGAACACAGCCAAT-3' NM_011697 537–701 165
  R: 5'-GGAGTGGGATGGATGATGTC-3'    
VEGF-C F: 5'-GGGAAGAAGTTCCACCATCA-3' NM_009506 1258–1392 135
  R: 5'-ATGTGGCCTTTTCCAATACG-3'    
VEGF-D F: 5'-GCTGTCACTGTTGCCCACTA-3' NM_010216 1423–1611 207
  R: 5'-CCCTTCCTTTCTGAGTGCTG-3'    
PLGF F: 5'-GGGAAGAAGCAAGACATGGA-3' NM_008827 1055–1261 189
  R: 5'-ATGTCCTGTCCCATCTCCAG-3'    
β-actin F: 5'-ACCCAGATCATGTTTGAGAC-3' X03765 280–503 224
  R: 5'-GTCAGGATCTTCATGAGGTAGT-3'    
  1. F, forward primer; PLGF, placental growth factor; R, reverse primer; VEGF, vascular endothelial growth factor.