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  • Research article
  • Open Access

Anti-cyclic citrullinated peptide antibodies in primary Sjögren syndrome may be associated with non-erosive synovitis

  • 1,
  • 1Email author,
  • 2,
  • 3,
  • 3,
  • 3 and
  • 3
Arthritis Research & Therapy200810:R51

  • Received: 15 November 2007
  • Accepted: 7 May 2008
  • Published:



The purpose of this study was to investigate the prevalence of cyclic citrullinated peptide antibodies (anti-CCP) in patients with primary Sjögren syndrome (pSS) and its correlation with clinical and laboratory data.


We analysed the clinical and serological data of 155 consecutive patients with pSS. Among these, 14 were excluded due to fulfillment of American College of Rheumatology criteria for rheumatoid arthritis (RA). So, 141 patients (27 males and 114 females; mean age 48 years, range 39 to 60) were clinically assessed for the presence of synovitis (objective swelling of one or more joints) and extra-glandular involvement. The anti-CCP antibodies were tested using a commercially available second-generation enzyme-linked immunosorbent assay. IgM rheumatoid factor (RF) was determined by nephelometry.


Fourteen patients (9.9%) had moderate to high levels of anti-CCP, and 94 (66.7%) were positive for RF. Eighty-one (57.4%) showed extra-glandular involvement, and 44 (31.2%) had synovitis without any radiographic sign of erosion. There was a close correlation between the presence of anti-CCP and synovitis (P < 0.001) but no association between anti-CCP and extra-glandular involvement (P = 0.77). Multivariate analysis confirmed the association between anti-CCP and an increased prevalence of synovitis (prevalence odds ratio for positive versus negative anti-CCP status 7.611, 95% confidence interval 1.475 to 74.870; P = 0.010).


Only a minority of patients with pSS are anti-CCP-positive, which seems to be closely associated with the prevalence of synovitis. Anti-CCP positivity in patients with pSS therefore may be a predictor of future progress to RA or an expression of the inflammatory process of synovial tissue.


  • Rheumatoid Arthritis
  • Rheumatoid Factor
  • Synovitis
  • Mixed Connective Tissue Disease
  • Keratoconjunctivitis Sicca


Primary Sjögren syndrome (pSS) is a chronic, slowly progressive, inflammatory, autoimmune disease that is characterised by lymphocytic infiltration of the exocrine glands (which reduces or eliminates glandular secretion) and marked B-lymphocytic cell hyper-reactivity, which initially is manifested by a variety of serum autoantibodies, including those against Ro (SSA) and La (SSB), and rheumatoid factor (RF) [14].

Most patients with pSS present only with keratoconjunctivitis sicca and xerostomia, but approximately 40% develop extra-glandular musculoskeletal manifestations; lung, kidney, and skin involvement; vasculitis; neuropathy; and lymphoma [1, 2]. The most common are arthralgia and an intermittent non-erosive polyarthropathy affecting mainly the small joints, which means that the picture may mimic that of rheumatoid arthritis (RA), particularly as 50% to 80% of cases are RF-positive [4, 5]. However, the fact that tests for cyclic citrullinated peptide antibodies (anti-CCP) usually are negative may help to differentiate the two conditions. Anti-CCP antibodies, which were first described in 1998, were found to be highly specific in the diagnosis of RA (95%) and only slightly less sensitive than IgM RF (60% to 70%) [6, 7], whereas the second generation of anti-CCP antibodies have a sensitivity of 80% and a specificity of 98%. Follow-up studies of patients with early RA have demonstrated that anti-CCP antibodies independently predict the development of erosions [8, 9], but Goëb and colleagues [10] found anti-CCP autoantibodies in only 4% of 137 women and 16% of 11 men with pSS.

Gottenberg and colleagues [11] studied a cohort of 134 patients with pSS and found that 7.5% of the serum samples were positive for anti-CCP antibodies and 5.2% were positive for anti-keratin antibodies (AKAs) without any radiographic evidence of erosion after a long follow-up. They suggested that the anti-CCP-positive patients, who may be prone to developing RA, require cautious clinical and radiographic follow-up. The aims of this study were to verify the prevalence of anti-CCP antibodies in patients with pSS and to investigate any associations with their clinical and laboratory characteristics.

Materials and methods


The study involved 155 consecutive SS patients who were evaluated at three tertiary rheumatologic referral centres and who fulfilled the American-European Consensus Group diagnostic criteria [12]. Fourteen patients with SS fulfilling the American College of Rheumatology (ACR) criteria for RA and/or presenting at least one joint erosion [13] were excluded; 141 patients (114 women and 27 men; mean age 48 years, range 39 to 60; mean disease duration 0.98 ± 6.12 years) were considered for the present study. ACR criteria were considered to be fulfilled if at least four criteria were present simultaneously for at least 6 weeks. All of the study subjects had their medical history recorded and underwent a clinical assessment, including 'synovitis' (defined as objective swelling of one or more joints in the absence of erosions) and extra-glandular involvement, erythrocyte sedimentation rate (ESR), C-reactive protein, RF, and anti-CCP, anti-extractable nuclear antigen (ENA), and antinuclear antibodies (ANAs). Hand and foot radiographs were also evaluated. Written informed consent was obtained from the patients before their inclusion in the study, which was approved by the ethics committees of the participating centres. All of the patients with synovitis and anti-CCP antibodies were re-evaluated by an experienced rheumatologist to definitively exclude a diagnosis of RA according to ACR criteria. To be classified as having RA, patients had to have met four criteria simultaneously for at least 6 weeks by the time of the evaluation or in the past.

Detection of anti-cyclic citrullinated peptide antibodies

Anti-CCP antibodies were tested using a commercially available second-generation enzyme-linked immunosorbent assay (ELISA) kit (Axis-Shield, Dundee, UK) as previously described [14]. The serum samples were evaluated in triplicate, with the upper normal limit of 5 IU/mL being assumed in accordance with the manufacturer's recommendations. Plates from the same batch (#470094) were used in order to avoid any plate-to-plate variations in anti-CCP measurements. Inter- and intra-assay variability was less than 9%.

Detection of rheumatoid factor

IgM RF was measured by means of immunonephelometry using the quantitative N Latex RF system (Dade Behring, now part of Siemens AG, Munich, Germany). Concentrations of greater than 15 IU/mL were considered positive.

Detection of antinuclear and anti-extractable nuclear antigen antibodies

ANAs were tested by means of standard indirect immunofluorescence, as previously described [14], using a BX 51 Olympus fluorescence microscope (Olympus Optical Co., Hamburg, Germany) at ×40 power. ENA antibodies were evaluated in triplicate using commercially available ELISA kits (Axis-Shield) according to the manufacturer's recommendations. The following individual ENA specificities were investigated: Sm, RNP, SSA (Ro), SSB (La), Scl-70, and Jo1.

Radiographic assessment

Radiographs of the hands and feet were evaluated at the time of the anti-CCP analysis. Patients with at least one erosion were excluded from the study.

Statistical analysis

The data were statistically analysed by means of R software, version 2.5 [15], using the Wilcoxon non-parametric rank sum test for continuous variables, and the Fisher exact or Pearson chi-square test with Yates' continuity correction for discrete variables. All of the analyses were two-tailed. P values of less than 0.05 were considered as indicating statistical significance. The variables found to be significantly associated with the prevalence of anti-CCP positivity at the univariate level were examined in a logistic regression model using exact inference (LogXact version 7; Cytel Inc., Cambridge, MA, USA). The prevalence odds ratio (OR) was used as the effect measure in this prevalence study [16].


Fourteen (9.9%) of the patients with pSS had moderate to high levels of anti-CCP antibody, and 94 (66.7%) were RF-positive, 134 (95%) ANA-positive, 115 (81.6%) SSA-positive, and 55 (39%) SSB-positive (Table 1). The mean value for anti-CCP antibodies was 46 IU/mL (28 to 78), with a normal range (as defined by the manufacturer) of less than 5 IU/mL. None of the 14 patients fulfilled the ACR criteria for RA. The focus score at lip biopsy was greater than 1 in 94 patients (67.1%). Extra-glandular involvement was found in 81 patients (57.4%) (Table 2), and synovitis was found in 44 (31.2%) without any radiographic evidence of erosions (Table 1).
Table 1

Demographic and clinical features of 141 patients with primary Sjögren syndrome







Age (range), years

48 (39–60)


Median disease duration (IQR), years

2.49 (0.98–6.12)


Synovitis (objective swelling)



Extra-glandular involvement (skin, lung, kidney, or vasculitis)



Median erythrocyte sedimentation rate (IQR), mm/1st hour

27 (17–38)


Median C-reactive protein (IQR), mg/dL

0.57 (0.31–0.9)


Rheumatoid factor



Anti-CCP antibodies



Anti-nuclear antibodies



Anti-La (SSB) antibodies



Anti-Ro (SSA) antibodies



Focus score of greater than 1



Demographic characteristics and continuous data are expressed as median (interquartile range, IQR). Categorical data are expressed as absolute frequency and percentage. Anti-CCP, cyclic citrullinated peptide antibody.

Table 2

Extra-glandular involvement in 141 patients with primary Sjögren syndrome subdivided for the specific manifestations




Raynaud phenomenon






Cutaneous vasculitis



Lung involvement



Kidney involvement



Liver involvement



Peripheral neuropathy









Rheumatoid factor-positive primary Sjögren syndrome patients

There were no correlations between RF titres and synovitis (P = 0.65), extra-glandular involvement (P = 0.85), laboratory parameters, or focus scores, and there were no significant differences between the RF-positive and RF-negative patients in terms of age (P = 0.26) or disease duration (P = 0.55).

Anti-CCP-positive primary Sjögren syndrome patients

There was a close relationship between anti-CCP positivity and synovitis (P < 0.001) but not between anti-CCP positivity and other extra-glandular involvement (P = 0.77). There were no significant differences between the anti-CCP-positive and -negative patients in terms of age (P = 0.13), disease duration (P = 0.41), mean ESR (P = 0.10), the presence of anti-SSA (P = 0.29), anti-SSB (P = 0.78), or RF (P = 0.77) or a focus score of greater than 1 (P = 0.77) (Table 3).
Table 3

Correlations with clinical and laboratory parameters of anti-CCP antibody-positive and -negative primary Sjögren syndrome patients


Anti-CCP-positive (n = 14)

Anti-CCP-negative (n = 127)

P value

Age (range), years

41 (34.5–52.2)

49 (39–60)


Disease duration, years

2.4 (1.40–7.5)

2.5 (0.98–5.2)


Synovitis, number (percentage)

12 (85.7)

32 (25.2)


Extra-glandular involvement, number (percentage)

9 (64.3)

72 (56.7)


Erythrocyte sedimentation rate (range), mm/1st hour

34 (24.25–45.5)

27 (16.5–38)


Rheumatoid factor, number (percentage)

10 (71.4)

84 (66.1)


Anti-nuclear antibodies, number (percentage)

13 (92.9)

121 (95.3)


Anti-La (SSB) antibodies, number (percentage)

10 (71.4)

105 (82.7)


Anti-Ro (SSA) antibodies, number (percentage)

6 (42.9)

49 (38.6)


Focus score of greater than 1, number (percentage)

9 (64.3)

85 (67.5)


Anti-CCP, cyclic citrullinated peptide antibody.

Multivariable analysis of synovitis

An exact logistic regression analysis was used to model the effects of the presence of positive anti-CCP antibodies on the prevalence of synovitis, adjusting for the potential covariates of age, disease duration, gender, and anti-SSA and anti-SSB antibodies. Age and disease duration were divided into quartiles with the aim of simplifying the interpretation of the results by comparing the oldest patients and those with the longest disease duration. Anti-SSA and anti-SSB antibodies were included in the model because they were found in 60% of the patients and are associated with more frequent extra-glandular manifestations [4, 17]. As shown in Table 4, multivariate analysis of the anti-CCP-positive patients, adjusted for age, gender, disease duration, and anti-SSA and anti-SSB antibodies, confirmed the association with an increased prevalence of synovitis (prevalence OR versus anti-CCP-negative patients = 7.611, 95% confidence interval [CI] 1.475 to 74.870; P = 0.010). None of the covariables included in the model was statistically significant except for anti-SSB antibodies, which were of borderline significance (prevalence OR versus anti-SSB-negative patients = 0.393, 95% CI 0.130 to 0.967; P = 0.084). Diagnostic procedures did not reveal any violations of the model assumptions, and the test of the deviance did not provide any evidence requiring the rejection of the null hypothesis of goodness-of-fit (P > 0.23).
Table 4

Multivariable associations between patient characteristics and the prevalence of synovitis

Model term


95% CI for OR

P value










Disease duration, years






   1 to <2.5




   2.5 to <6




   ≥ 6



Age, years






   40 to <50




   50 to <60




   ≥ 60






























Anti-CCP, cyclic citrullinated peptide antibody; CI, confidence interval; OR, prevalence odds ratio.


Musculoskeletal manifestations such as fatigue, myalgia, arthralgia, an intermittent non-erosive polyarthropathy affecting mainly the small joints, and usually mild synovitis are common in patients with pSS and may mimic RA, particularly in the presence of RF [2, 3, 18], although the fact that tests for anti-CCP antibodies usually are negative may help to differentiate the two conditions [19, 20].

We found slightly high anti-CCP levels in 14 of our 141 patients with pSS (9.9%). Some studies have found that anti-CCP antibodies are an independent factor predicting the development of erosions in patients with RA [8, 9], but none of our 44 pSS patients with synovitis (31.2%) showed any radiographic sign of erosions. After a long follow-up, Gottenberg and colleagues [11] found that 80 out of 134 pSS patients with no radiographic evidence of erosions (59%) were positive for IgM RF, 10 (7.5%) for anti-CCP, 7 (5.2%) for AKA, and 5 (3.7%) for both anti-CCP and AKA.

We also found no significant differences between our anti-CCP-positive and -negative patients in terms of demographic factors or extra-glandular manifestations. However, the presence of synovitis in pSS patients did seem to be closely associated with the presence of anti-CCP antibodies as the odds of having synovitis were 1.475 times greater in our anti-CCP-positive patients, regardless of the other covariables in the model. There were no other significant associations with clinical or laboratory parameters between the anti-CCP-positive and -negative patients.

In addition, anti-SSB positivity was weakly and inversely associated with the prevalence of synovitis. Antibodies to the Ro/SSA and La/SSB ribonucleoprotein particles usually are found in the serum of pSS patients and are associated with a longer disease duration, more frequent non-exocrine manifestations, and more intense lymphocytic infiltration of the minor salivary glands [21]. The prevalence of anti-SSB is higher in pSS, but in our patient cohort it was also inversely related to the presence of synovitis.

The possibility that our anti-CCP patients represent a subgroup of patients with RA and secondary SS was ruled out by excluding patients fulfilling ACR criteria at the beginning of the study and re-evaluating all of the anti-CCP-positive patients. In our study, we decided to be very stringent in the application of ACR criteria, and we classify as RA those patients in whom clinical symptoms (symmetrical arthritis, arthritis of the hands, more than three groups of joints involved, and morning stiffness) were simultaneously present at the moment of evaluation or for at least 6 weeks in the past. In fact, even if we had considered the possibility of a diagnosis of RA for symptoms that were successive and not simultaneously present, none of the 14 patients would have been classifiable as having RA (data not shown).

Indeed, the possibility that patients with anti-CCP antibodies may develop RA cannot be ruled out by the present study, and it is possible that in the future anti-CCP will be interpreted as an additional marker of definite RA. It should be noted, however, that anti-CCP antibodies are strictly associated with the development of erosions in RA and that none of our patients developed erosions after a mean follow-up of 2.4 years.

As a matter of fact, anti-CCP antibodies may also be observed in a minority of patients with other systemic autoimmune diseases. Takasaki and colleagues [22] detected anti-CCP antibodies in patients with mixed connective tissue disease (MCTD) (9%), systemic lupus erythematosus (14%), systemic sclerosis (13%), polymyositis/dermatomyositis (14%), and SS (18%). Some of these patients presented overlapping RA and connective tissue diseases (particularly MCTD) and so, as in the case of other autoimmune diseases, anti-CCP positivity in pSS may be a marker of synovial tissue inflammation. The results of our study suggest that the production of anti-CCP antibodies may be less related to the pathogenesis of RA. It is possible that marked B-lymphocyte hyper-reactivity (a characteristic of pSS) may explain the presence of anti-CCP antibodies, as usually is observed in the case of RF and anti-SSA and anti-SSB antibodies [23].


Only a minority of patients with pSS are anti-CCP-positive, but the prevalence of synovitis seems to be closely associated with the presence of anti-CCP antibodies as multivariate analysis confirmed the association between anti-CCP positivity and an increased prevalence of synovitis. However, the possibility that patients with anti-CCP antibodies may develop RA cannot be ruled out, particularly in patients with the concomitant presence of IgM RF.



= American College of Rheumatology


= anti-keratin antibody


= antinuclear antibody


= cyclic citrullinated peptide antibody


= confidence interval


= enzyme-linked immunosorbent assay


= anti-extractable nuclear antigen


= erythrocyte sedimentation rate


= mixed connective tissue disease


= odds ratio


= primary Sjögren syndrome


= rheumatoid arthritis


= rheumatoid factor


= Sjögren syndrome.


Authors’ Affiliations

Rheumatology Unit, L. Sacco Hospital, University of Milan, Via G.B. Grassi 74, 20127 Milan, Italy
Department of Medicine and Public Health, Second University of Naples, Via L Armanni 75, 80139 Naples, Italy
University of Pavia, IRCCS Policlinico S. Matteo, Piazzale Golgi 12, 27100 Pavia, Italy


  1. Fox PC: Autoimmune diseases and Sjögren's syndrome: an autoimmune exocrinopathy. Ann N Y Acad Sci. 2007, 1098: 15-21. 10.1196/annals.1384.003.View ArticlePubMedGoogle Scholar
  2. Papiris SA, Tsonis IA, Moutsopoulos HM: Sjögren's syndrome. Semin Respir Crit Care Med. 2007, 28: 459-471. 10.1055/s-2007-985667.View ArticlePubMedGoogle Scholar
  3. Rehman HU: Sjögren's syndrome. Yonsei Med J. 2003, 44: 947-954.View ArticlePubMedGoogle Scholar
  4. Manoussakis MN, Tzioufas AG, Pange PJ, Moutsopoulos HM: Serological profiles in subgroups of patients with Sjögren's syndrome. Scand J Rheumatol Suppl. 1986, 61 (Suppl): 89-92.PubMedGoogle Scholar
  5. van Paassen P, Damoiseaux J, Tervaert JW: Laboratory assessment in musculoskeletal disorders. Best Pract Res Clin Rheumatol. 2003, 17: 475-494. 10.1016/S1521-6942(03)00029-9.View ArticlePubMedGoogle Scholar
  6. Schellekens GA, de Jong BA, Hoogen van den FH, Putte van de LB, van Venrooij WJ: Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis-specific autoantibodies. J Clin Invest. 1998, 101: 273-281. 10.1172/JCI1316.PubMed CentralView ArticlePubMedGoogle Scholar
  7. Van Gaalen FA, Linn Rasker SP, Venrooij WJ, de Jong BA, Breedveld FC, Verweij CL, Toes RE, Huizinga TW: Autoantibodies to cyclic citrullinated peptides predict progression to rheumatoid arthritis in patients with undifferentiated arthritis. A prospective cohort study. Arthritis Rheum. 2004, 50: 709-715. 10.1002/art.20044.View ArticlePubMedGoogle Scholar
  8. Meyer O, Labarre C, Dougados M, Goupille P, Cantagrel A, Dubois A, Nicaise-Roland P, Sibilia J, Combe B: Anticitrullinated protein/peptide antibody assays in early rheumatoid arthritis for predicting five year radiographic damage. Ann Rheum Dis. 2003, 62: 120-126. 10.1136/ard.62.2.120.PubMed CentralView ArticlePubMedGoogle Scholar
  9. Vencovsky J, Machacek S, Sedova L, Kafkova J, Gatterova J, Pesakova V, Růzicková S: Autoantibodies can be prognostic markers of an erosive disease in early rheumatoid arthritis. Ann Rheum Dis. 2003, 62: 427-430. 10.1136/ard.62.5.427.PubMed CentralView ArticlePubMedGoogle Scholar
  10. Goëb V, Salle V, Duhaut P, Jouen F, Smail A, Ducroix JP, Tron F, Le Loët X, Vittecoq O: Clinical significance of autoantibodies recognizing Sjögren's syndrome A (SSA), SSB, calpastatin and alpha-fodrin in primary Sjögren's syndrome. Clin Exp Immunol. 2007, 148: 281-287.PubMed CentralView ArticlePubMedGoogle Scholar
  11. Gottenberg JE, Mignot S, Nicaise-Rolland P, Cohen-Solal J, Aucouturier F, Goetz J, Aucouturier F, Goetz J, Labarre C, Meyer O, Sibilia J, Mariette X: Prevalence of anti-cyclic citrullinated peptide and anti-keratin antibodies in patients with primary Sjögren's syndrome. Ann Rheum Dis. 2005, 64: 114-117. 10.1136/ard.2003.019794.PubMed CentralView ArticlePubMedGoogle Scholar
  12. Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander EL, Carsons SE, Daniels TE, Fox PC, Fox RI, Kassan SS, Pillemer SR, Talal N, Weisman MH, European Study Group on Classification Criteria for Sjögren's Syndrome: Classification criteria for Sjögren's syndrome: a revised version of the European criteria proposed by the American-European Consensus Group. Ann Rheum Dis. 2002, 61: 554-558. 10.1136/ard.61.6.554.PubMed CentralView ArticlePubMedGoogle Scholar
  13. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, et al: The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988, 31: 315-324. 10.1002/art.1780310302.View ArticlePubMedGoogle Scholar
  14. Bobbio-Pallavicini F, Alpini C, Caporali R, Avalle S, Bugatti S, Montecucco C: Autoantibody profile in rheumatoid arthritis during long-term infliximab treatment. Arthritis Res Ther. 2004, 6: R264-272. 10.1186/ar1173.PubMed CentralView ArticlePubMedGoogle Scholar
  15. R Development Core Team: R: A Language and Environment for Statistical Computing. 2005, Vienna, Austria: R Foundation for Statistical Computing, ISBN 3-900051-07-0.Google Scholar
  16. Pearce N: Effect measures in prevalence studies. Environ Health Perspect. 2004, 112: 1047-1050.PubMed CentralView ArticlePubMedGoogle Scholar
  17. Nakamura H, Kawakami A, Eguchi K: Mechanisms of autoantibody production and the relationship between autoantibodies and the clinical manifestations in Sjögren's syndrome. Transl Res. 2006, 148: 281-288. 10.1016/j.trsl.2006.07.003.View ArticlePubMedGoogle Scholar
  18. Castro-Poltronieri A, Alarcón-Segovia D: Articular manifestations of primary Sjögren's syndrome. J Rheumatol. 1983, 10: 485-488.PubMedGoogle Scholar
  19. Rantapaa-Dahlqvist SR, de Jong BA, Berglin E, Hallmans G, Wadell G, Stenlund H, Sundin U, van Venrooij WJ: Antibodies against cyclic citrullinated peptides and IgA rheumatoid factor predict the development of rheumatoid arthritis. Arthritis Rheum. 2003, 48: 2741-2749. 10.1002/art.11223.View ArticlePubMedGoogle Scholar
  20. Kamali S, Polat NG, Kasapoglu E, Gul A, Ocal L, Aral O, Konice M, Badur S, Inanc M: Anti-CCP and antikeratin antibodies in rheumatoid arthritis, primary Sjögren's syndrome, and Wegener's granulomatosis. Clin Rheumatol. 2005, 24: 673-676. 10.1007/s10067-005-1104-y.View ArticlePubMedGoogle Scholar
  21. Tzioufas AG, Wassmuth R, Dafni UG, Guialis A, Haga HJ, Isenberg DA, Jonsson R, Kalden JR, Kiener H, Sakarellos C, Smolen JS, Sutcliffe N, Vitali C, Yiannaki E, Moutsopoulos HM: Clinical, immunological, and immunogenetic aspects of autoantibody production against Ro/SSA, La/SSB and their linear epitopes in primary Sjögren's syndrome (pSS): a European multicentre study. Ann Rheum Dis. 2002, 61: 398-404. 10.1136/ard.61.5.398.PubMed CentralView ArticlePubMedGoogle Scholar
  22. Takasaki Y, Yamanaka K, Takasaki C, Matsushita M, Yamada H, Nawata M, Matsudaira R, Ikeda K, Kaneda K, Hashimoto H: Anticyclic citrullinated peptide antibodies in patients with mixed connective tissue disease. Mod Rheumatol. 2004, 14: 367-375. 10.1007/s10165-004-0325-2.View ArticlePubMedGoogle Scholar
  23. Jonsson R, Nginamau E, Szyszko E, Brokstad KA: Role of B cells in Sjögren's syndrome – from benign lymphoproliferation to overt malignancy. Front Biosci. 2007, 12: 2159-2170. 10.2741/2219.View ArticlePubMedGoogle Scholar


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